Gene Modulation Services Shared Resource (GMSR)

What we do

The Gene Modulation Services Shared Resource (GMSR), a partnership between Roswell Park Comprehensive Cancer Center and the State University of New York at Buffalo (UB), provides a centralized, cost‐effective service supporting investigators interested in modulating gene expression.

The resource houses individual short hairpin RNAs (shRNAs) in retro- or lentivirus vectors (140,000 for human and 84,000 for analog genes), pooled genomic shRNA lentivirus screening libraries, pooled CRISPR-inhibitor or CRISPR-activator sgRNA lentivirus libraries and associated dCas9 regulators, the human ORFeome 8.1 lentivirus library with 13.5K individual full-length cDNA clones, and various virus vectors expressing selection markers (e.g., GFP or puromycin-resistance) or immortalization genes.

Technology

Expression Arrest pGIPZ Lentiviral genome-wide Human shRNA Library and the Expression Arrest pSM2 Retroviral genome-wide Human and Mouse shRNA Libraries

These libraries, purchased from Thermo Fisher (at the time Open Biosystems), contain ~62K unique shRNAmir constructs targeting the entire genomes (>25,000 genes) with ~3-5 constructs/target.

More information on these libraries is available on Dharmacon's website and Drexel's website.

Expression Arrest pSM2 Retroviral shRNAmir Human Cancer shRNA Library

2311 shRNAmir constructs targeting 967 human cancer-related genes.

Dharmacon™ Decode™ Pooled Human Lentiviral shRNA Screening Library

Decode lentiviral RNAi screening libraries are pools of Dharmacon™ GIPZ™ shRNAs. Through RNAi-mediated silencing of hundreds or thousands of genes in parallel, a pooled lentiviral shRNA screen can be performed to identify genes that regulate cellular responses and signaling pathways, or to discover novel gene functions.

For more information on this library, please see Dharmacon's website.

DECIPHER™ Pooled shRNA Human and Mouse Lentiviral Libraries from CELLECTA

These barcoded lentiviral shRNA were made to cover the entire annotated human and mouse gene sets with a high percentage of functionally validated sequences and inclusion of a redundant set of five to six shRNA per gene. The DECIPHER libraries are broken down into modules: three modules for human and two modules for mice. Each module targets roughly 5,000 genes ̶ variously associated with diseases, known drug targets, or signaling pathways ̶ and represents over 25,000 unique shRNAs. The libraries use optimized 18-nt barcodes that facilitate the identification of functional shRNAs and high-throughput sequencing data analysis using the Illumina platform.

For more information of these libraries, please see Cellecta's website.

MGC premier Human Lentiviral ORF Library

This human Lentiviral ORF Expression Library was developed through the collaborative efforts of Dana-Farber Cancer Institute, The Broad Institute and the Center for Cancer Systems Biology (CCSB). The collection includes 15597 clones covering 12861 genes.

For more information of this libraries, please see Transomic's website.

Human genome-scale CRISPRi and CRISPRa Pooled Libraries

CRISPRa single guide RNA (sgRNA) library from the Jonathan Weissman Lab (Version 1)

Featuring the SunCas9 system with 10 sgRNAs for each transcription start site (TTS) in 15,977 human genes and 5,968 controls for a total of 198,810 total sgRNAs
Constitutive or Tetracycline regulated systems available
190X coverage

CRISPRi sgRNA library from the Jonathan Weissman Lab (Version 1)

10 sgRNAs for each TSS in 15,977 human genes and 11,219 controls for a total of 206,421 total sgRNAs
290X coverage

PCR based mycoplasma testing for routine monitoring of tissue culture lines
Retro- or lentiviral packaging of user-provided constructs
Production of infectious virus from additional core-provided vectors, including those for cellimmortalization, TET regulation and tracking
CsCl based plasmid DNA maxiprep

Protocols

View SOP for adherent cell infection with Retro or Lentivirus

View SOP for suspension cell infection with Retro or Lentivirus

View SOP for Lentiviral Packaging

View SOP for Retroviral Packaging

Services & fees

Product or service	1-5 Constructs	>5 Constructs
Glycerol stock – shRNA or ORF	$52.50	$26
Plasmid DNA – shRNA or ORF	$77	$61
Virus production for infection of cell line	$118	$94
Infection of cell line and antibiotic selection	$161	$112
Mycoplasma testing	$37.50	$20
CsCl purified plasmid DNA maxiprep	$246.50	$145
Bacterial transformation	$55.50	$54.50

^* Plasmid DNA from Pooled shRNA or CRISPR libraries – please contact for pricing

^* High Titer Lentiviral Supernatant from Pooled shRNA or CRISPR libraries – please contact for pricing

Place an order

Mycoplasma testing

Mycoplasma testing order form

Individual shRNA clones

To place an order, you will need the official gene name. This is available at the NCBI website.
In the search window select “gene” and type gene name or alias.
To find available shRNA constructs for your gene of interest, visit this link. You can search either by official gene name or by accession number. You will need to supply the clone ID and plate address.
Requests for clones and questions about pricing and availability can be sent to renae.holtz@roswellpark.org.

Individual shRNA clone order form

PI supplied plasmids

PI supplied plasmids order form

LentiORF clones

To place an order, you will need the official gene name. This is available at the NCBI website.
In the search window select “gene” and type gene name or alias.
To find available LentiORF constructs for your gene of interest, visit this link.
You can search either by official gene name or by accession number. You will need to supply the clone ID and plate address.
Requests for clones and questions about pricing and availability can be sent to renae.holtz@roswellpark.org.

LentiORF clone order form

Tracking plasmids such as Luciferase or GFP

Plasmid tracking order form

CsCl plasmid production

CsCl plasmid production order form

Location & Hours

Roswell Park Comprehensive Cancer Center
Gene Modulation Services Resource
Center for Genetics and Pharmacology (CGP), Room L2-135
Elm and Carlton Streets
Buffalo, NY 14263

Monday — Friday, 8:30 a.m. - 4:30 p.m.

Please contact:

Irwin Gelman, PhD
Director
Phone: 716-845-7681
Email: Irwin.Gelman@RoswellPark.org