What we do

The Bioanalytics, Metabolomics & Pharmacokinetics (BMPK) Shared Resource supports research and preclinical/clinical drug development at Roswell Park Comprehensive Cancer Center in addition to providing bioanalytical, modeling and consultation services to other cancer centers, academic institutions and industry.

BMPK provides state-of-the-art bioanalytical data and PK results to investigators from their research and clinical studies of cancer therapeutics and preventive agents. In addition, BMPK advises investigators on PK/PD study design along with sample collection, handling and dosing selection recommendations in order to optimize the therapeutic outcomes from their investigations.

Using cutting-edge techniques with LC-MS/MS, UPLC, HPLC, and atomic absorption spectrophotometry, BMPK provides highly sensitive measurements for an extensive array of chemotherapeutic agents and metabolites.

Tools and equipment

Piece of science equipment in the BMPK lab

BMPK instrumentation

  • Applied Biosystems 5500 QTrap triple quadrupole (ESI/APCI-LC/MS/MS)
  • Applied Biosystems 5500 triple quadrupole (ESI/APCI-LC/MS/MS)
  • Thermo Scientific TSQ Vantage triple quadrupole (ESI/APCI-LC/MS/MS)
  • Applied Biosystems API3000 triple quadrupole (ESI LC/MS/MS)
  • Waters Ultra Performance Liquid Chromatography (UPLC) system with fluorescence and UV detection
  • Waters BioAlliance High Performance Liquid Chromatography (HPLC) system with fluorescence and UV detection
  • Abbott i1000SR Architect chemiluminescent magnetic microparticle immunoassay (CMIA)
  • Perkin Elmer Analyst 600 graphite furnace atomic absorption
  • Biotek Synergy 96-well plate reader providing fluorescence and UV detection
  • Tomtec Quadra 4 robotic handling system
Two medical professionals using the BMPK Modeling Software

BMPK modeling software

  • Phoenix WinNonlin: Non-compartmental and compartmental modeling
  • NONMEM: Nonlinear mixed effect modeling software for population analysis to determine sources of variability in the PK and PD of drugs
  • ADAPT 5 and S-ADAPT: individual compartmental PK and PK/PD modeling
  • SAS 9.3: used to manage and clean databases, and create graphics and tables

Services

BMPK is available to assist investigators in the design of their clinical and research studies incorporating clinical pharmacology, pharmacokinetic and pharmacodynamic objectives. Emphasis is placed on designing studies that optimize efficiency and scientific rigor.

To achieve these objectives, BMPK assists with the development and validation/qualification of new assay methodologies, sample analysis and PK/PD data analysis and modeling. Investigators are encouraged to consult BMPK as early as possible during the planning phases of their project or grant development.

Example categories of drug/metabolite and other small molecule assays:

BMPK Team Member using science equipment
  • Hormones: Testosterone, dihydrotestosterone, androstenedione, dehydroepiandrosterone (DHEA), and androsterone (LC-MS/MS), and estrone and estradiol (LC-MS/MS)
  • Antimetabolites: Gemcitabine and dFdU (LC-MS/MS), capecitabine (LC-MS/MS), and 5-fluorouracil (LC-MS/MS)
  • Taxanes: Docetaxel (LC-MS/MS) and paclitaxel (LC-MS/MS)
  • Topoisomerase Agents: Irinotecan (CPT-11), SN-38, SN-38G (UPLC and LC-MS/MS)
  • Anthracyclines: Doxorubicin (UPLC) and daunorubicin (UPLC)
  • Targeted Agents: Tivozanib (LC-MS/MS), enzalutamide and N-desmethylenzalutamide (LC-MS/MS), sorafenib and sorafenib-N-oxide (LC-MS/MS), carfilzomib (LC-MS/MS), sunitinib (LC-MS/MS), dabrafenib (LC-MS/MS), abemaciclib and abemaciclib M2 (LC-MS/MS)
  • Platinum Based Compounds: Oxaliplatin (atomic absorption), cisplatin (atomic absorption), and carboplatin (atomic absorption)
  • Selenium Based Compounds: Selenomethionine and methylselenocysteine (atomic absorption)
  • Others: Tryptophan/kynurenine (LC-MS/MS), sex hormone binding globulin (SHBG; CMIA), VEGFR2 (ELISA), lignans (enterodiol and enterolactone; LC-MS/MS) and eicosanoids (LC-MS/MS)
  • For a complete listing and additional specifications, refer to the table below.
Analyte(s) Lower Limit of Quantitation
(LLOQ)
Calibration Range Methodology Status Matrices
Androgens (5)
Androstenedione
Testosterone
Dihydrotestosterone Dehydroepiandrosterone
Androsterone
6.25 pg/mL
6.25 pg/mL
12.5 pg/mL
200 pg/mL
200 pg/mL
0.00625 - 3.75 ng/mL
0.00625 - 3.75 ng/mL
0.0125 - 7.50 ng/mL
0.200 - 7.50 ng/mL
0.200 - 7.50 ng/mL
LC-MS/MS Validated Mouse Serum
Human Heparinized Plasma
Human Serum

Biocrates (630 Metabolites)

Variable Variable LC-MS/MS Kit-Based Assay Variable
Calcitriol 10.0 ng/mL 10.0 - 1,000 ng/mL LC-MS/MS Validated Drug Formulation
Carfilzomib 0.400 ng/mL 0.400 - 1,000 ng/mL LC-MS/MS Validated Human Heparinized Plasma
Ceritinib 1.00 ng/mL 1.00 - 500 ng/mL LC-MS/MS Validated Human Plasma
CFAK-C4 2.50 pg/mL 2.50 - 2,500 pg/mL LC-MS/MS Qualified Dog Heparinized Plasma
CPT-11 (Irinotecan) 2.50 ng/mL 2.50 - 1,000 ng/mL UPLC / Fluorescence Qualified Human Plasma
Cytarabine 1.00 ng/mL 1.00 - 1,000 ng/mL LC-MS/MS Qualified Human Plasma
Docetaxel 0.200 ng/mL 0.200 - 400 ng/mL LC-MS/MS Validated Human Plasma
Docetaxel
Docetaxel M1
Docetaxel M2
Docetaxel M3
Docetaxel M4
5.00 ng/mL
5.00 ng/mL
5.00 ng/mL
5.00 ng/mL
7.50 ng/mL
5.00 - 1,000 ng/mL
5.00 - 1,000 ng/mL
5.00 - 1,000 ng/mL
5.00 - 1,000 ng/mL
7.50 - 1,500 ng/mL
LC-MS/MS Qualified Mouse Plasma
Rat Plasma
Dog Plasma
Human Plasma
Dovitinib 2.00 ng/mL 2.00 - 1,000 ng/mL LC-MS/MS Qualified Human Heparinized Plasma
Eicosanoids (7)
Prostaglandin D2
Prostaglandin E2
13-Hydroxyoctadecadienoic acid
5-Hydroxyeicosatetraenoic acid
12-Hydroxyeicosatetraenoic acid
15-Hydroxyeicosatetraenoic acid
Arachidonic Acid
1.00 ng/mL 1.00 - 400 ng/mL LC-MS/MS Qualified Mouse Xenografts
Enterodiol
Enterolactone
0.500 ng/mL
0.500 ng/mL
0.500 - 150 ng/mL
0.500 - 150 ng/mL
LC-MS/MS Validated Human Serum
Entinostat 0.500 ng/mL 0.500 - 250 ng/mL LC-MS/MS Qualified Human Plasma
Enzalutamide
N-Desmethyl Enzalutamide
10.0 ng/mL
10.0 ng/mL
10.0 - 20,000 ng/mL
10.0 - 20,000 ng/mL
LC-MS/MS Validated Human EDTA Plasma
Enzalutamide
N-Desmethyl Enzalutamide
10.0 ng/mL
10.0 ng/mL
10.0 - 20,000 ng/mL
10.0 - 20,000 ng/mL
LC-MS/MS Qualified Woodchuck EDTA Plasma
Epacadostat 5.00 ng/mL 5.00 - 1,000 ng/mL LC-MS/MS Validated Human EDTA Plasma
Estrone and
17ß-Estradiol
2.00 pg/mL
1.00 pg/mL
2.00 - 250 pg/mL
1.00 - 250 pg/mL
LC-MS/MS Validated Human Heparinized Plasma
Human Serum
FL118 1.00 ng/mL 1.00 - 500 ng/mL UPLC / Fluorescence Qualified Human Plasma
Gemcitabine
dFdU
1.00 ng/mL
5.00 ng/mL
1.00 - 2,000 ng/mL
5.00 - 10,000 ng/mL
LC-MS/MS Validated Human Plasma
Gemcitabine
dFdU
Capecitabine
5-Fluorouracil
5-DFCR
5-DFUR
1.00 ng/mL
1.00 ng/mL
1.00 ng/mL
5.00 ng/mL
5.00 ng/mL
5.00 ng/mL
1.00 - 1,000 ng/mL
1.00 - 1,000 ng/mL
1.00 - 1,000 ng/mL
5.00 - 1,000 ng/mL
5.00 - 1,000 ng/mL
5.00 - 1,000 ng/mL
LC-MS/MS Qualified Human Plasma
Irinotecan (CPT-11) 2.50 ng/mL 2.50 - 1,000 ng/mL UPLC / Fluorescence Qualified Human Plasma
JP-11646 1.00 ng/mL 1.00 - 500 ng/mL LC-MS/MS Validated Mouse Plasma
Rat Plasma
Dog Plasma
Mannitol
Lactulose
3-MGP
0.980 µg/mL
0.980 µg/mL
2.93 µg/mL
0.980 - 62.5 µg/mL
0.980 - 62.5 µg/mL
2.93 - 62.5 µg/mL
LC-MS/MS Qualified Human Plasma
Molybdenum 10.0 ng/mL 10.0 - 1,000 ng/mL Atomic Absorption Qualified Human Plasma
Nicotine
Cotinine
Hydroxy Cotinine
Cotinine Glucuronide
Anatabine
Atabasine
1.00 ng/mL
1.00 ng/mL
1.00 ng/mL
10.0 ng/mL
1.00 ng/mL
1.00 ng/mL
1.00 - 100 ng/mL
1.00 - 100 ng/mL
1.00 - 100 ng/mL
10.0 - 500 ng/mL
1.00 - 100 ng/mL
1.00 - 100 ng/mL
LC-MS/MS Qualified Human Urine
Nintedanib 0.500 ng/mL 0.500 - 250 ng/mL LC-MS/MS Validated Human EDTA Plasma
Paclitaxel and
6α-Hydroxy Paclitaxel
2.00 ng/mL
2.00 ng/mL
2.00 - 1,000 ng/mL
2.00 - 1,000 ng/mL
LC-MS/MS Validated Human Plasma
Pemetrexed 5.00 ng/mL 5.00 - 2,000 ng/mL LC-MS/MS Qualified Human Plasma
Platinum 250 ng/mL 250 - 8,000 ng/mL Atomic Absorption Qualified Drug Formulation
Human Plasma
Prostate Specific Antigen (PSA) 100 pg/mL 0.100-100 ng/mL CMIA Validated Human and Mouse Serum
Rapamycin 1.25 ng/mL 1.25 - 100 ng/mL LC-MS/MS Validated Human Plasma
Selenium 70.0 ng/mL 70.0 - 1,200 ng/mL Atomic Absorption Validated Human Plasma
Sex Hormone Binding Globulin (SHBG) 2.00 nmol/L 2.00 - 250 nmol/L Chemiluminescent microparticle immunoassay Validated Human Serum
SN-38
SN-38G
0.200 ng/mL
0.200 ng/mL
0.200 - 1,000 ng/mL UPLC / Fluorescence Qualified Human Plasma
Sorafenib
Sorafenib N-oxide
50.0 ng/mL
12.5 ng/mL
50.0 - 10,000 ng/mL
12.5 - 2,500 ng/mL
LC-MS/MS Validated Human Plasma
Sorafenib
Sorafenib N-oxide
50.0 ng/mL
12.5 ng/mL
50.0 - 10,000 ng/mL
12.5 - 2,500 ng/mL
LC-MS/MS Qualified Woodchuck Plasma
(Z)-Tamoxifen
(Z)-N-Desmethyltamoxifen
(Z)-4-Endoxifen
(Z)-4-Hydoxytamoxifen
1.00 ng/mL
1.00 ng/mL
0.500 ng/mL
0.250 ng/mL
1.00 - 400 ng/mL
1.00 - 400 ng/mL
0.500 - 200 ng/mL
0.250 - 100 ng/mL
LC-MS/MS Validated Human Plasma
Human Breast Tissue
Tivozanib 0.500 ng/mL 0.500 - 150 ng/mL LC-MS/MS Validated Human EDTA Plasma
Tryptophan and Kynurenine 40.0 ng/mL
10.0 ng/mL
40.0 - 20,000 ng/mL
10.0 - 5,000 ng/mL
LC-MS/MS Validated Human Plasma
VEGFR2 80.0 pg/mL 80.0 - 5,000 pg/mL ELISA Validated Human EDTA Plasma

Fees

Collaborative interaction is essential to achieve optimal experimental design and outcomes. As a client of BMPK, we will arrange a one-on-one consultation with you to review your experimental design, method development needs, sample analysis strategy, and results interpretation in order to best meet your study aims and objectives. Over the course of the study, we will work closely with you to ensure that you receive the most pertinent and reliable outcomes possible. As a result, pricing varies with experimental design, turnaround time, the number of analytes as well as the number of samples.

When requesting assistance in obtaining/reviewing background information, and preparing a letter of support and budget, please give us at least two weeks advance notice before your deadline. Investigators who submit samples to BMPK are required to fill out a BMPK Sample Submission Form. Samples will not be analyzed without a completed sample submission form. Investigators interested in the use of the BMPK Shared Resource should contact Joshua Prey to discuss scheduling, shared resource procedures and pricing.

Contact Us

Joshua Prey, MS
Research Project Administrator
Phone: 716-845-1300, ext. 3313
Email: Joshua.Prey@RoswellPark.org

Mohammad Ghasemi, PhD
HRI Scientist, Pharmacokinetics & Pharmacodynamics
Phone: 716-845-1300, ext. 7844
Email: Mohammad.Ghasemi@RoswellPark.org

Image
Roswell Park Comprehensive Cancer Center

Location and hours

Roswell Park Comprehensive Cancer Center
Bioanalytics, Metabolomics & Pharmacokinetics Shared Resource
Center for Genetics and Pharmacology, Room L1-140
Elm and Carlton Streets
Buffalo, New York 14263

Monday - Friday, 8 a.m. - 5 p.m.

BMPK is partially funded by NCI P30CA16056. Authors should cite the core grant in the acknowledgment section of the publication if data that was generated by BMPK is used in the publication. Two copies of the publication, acknowledging the core grant, should be submitted to the facility at Elm and Carlton Streets, Buffalo NY, 14263.

For BMPK facility data, please click here.

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  • Hess TA, Drinkhouse ME, Prey JD, Miller JM, Fettig AA, Carberry CA, Brenn SH, Bailey DB. Analysis of platinum content in biodegradable carboplatin-impregnated beads and retrospective assessment of tolerability for intralesional use of the beads in dogs following excision of subcutaneous sarcomas: 29 cases (2011-2014). J Am Vet Med Assoc. 2018 Feb 15;252(4):448-456. doi: 10.2460/javma.252.4.448 PMID: 29393745
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  • Vicky C. Chang, Michelle Cotterchio, Beatrice A. Boucher, David J. A. Jenkins, Lucia Mirea, Susan E. McCann & Lilian U. Thompson (2018) Effect of Dietary Flaxseed Intake on Circulating Sex Hormone Levels among Postmenopausal Women: A Randomized Controlled Intervention Trial, Nutrition and Cancer, DOI: 10.1080/01635581.2018.1516789