Dhyan Chandra, PhD Dyhan Chandra, PhD

Dhyan Chandra

PhD

Special Interests:

Heat shock protein regulation of cancer cell death and survival Mitochondrial regulation of cell death in cancer Oxidative phosphorylation and reactive oxygen species Cancer health disparities Anticancer therapeutics and phytochemicals

About Dhyan Chandra

Positions

Roswell Park Comprehensive Cancer Center
  • Associate Professor of Oncology
  • Department of Pharmacology & Therapeutics
  • Member, Molecular Pharmacology and Cancer Therapeutics Graduate Program
  • Member, Experimental Therapeutics of CCSG (Comprehensive Cancer Support Grant) program
  • Member, Breast, Lung, Genitourinary, and Gastrointestinal DSRGs (Disease Site Research Groups)

Background

Education and Training:

  • PhD - School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
  • Postdoctoral - University of Texas MD Anderson Cancer Center, Smithville, Texas

Research

Research Overview:

The main focus of our research is to understand the molecular basis of therapy resistance in multiple cancer types including in prostate, breast, and colon cancers. To accomplish our goals, we are investigating two different, but complementary projects. The first project delineates how mitochondria-mediated cell death signaling is defective in cancer cells and cancer stem cells.

The second project defines the role of heat-shock proteins in cancer cell survival and death. We are also characterizing the role of mitochondria in health disparities among prostate and breast cancer patients. Our research suggests that protein complexes are important regulators of cancer cell death and survival. We use multiple biochemical, genetic, cellular, and molecular approaches to identify and characterize protein complexes in subcellular compartments including in the mitochondrion. Detailed understanding of protein complexes will lay a foundation for targeting cell death and survival machinery for cancer therapy. Our model system includes both laboratory cell culture and mouse model of cancer to examine cellular signaling in response to anticancer agents as well as phytochemicals. Our ultimate goal is to target mitochondria and cell death for prevention and therapy of multiple types of cancer.

The National Cancer Institute, American Cancer Society, and Department of Defense support our research.


Publications

Full Publications list on PubMed

Key Publications:

  • Yadav N, Pliss A, Kuzmin A, Rapali P, Sun L, Prasad P, Chandra D. Transformations of the macromolecular landscape at mitochondria during DNA-damage-induced apoptotic cell death. Cell Death Dis. 2014 Oct 9;5:e1453. doi: 10.1038/cddis.2014.405. PMID: 25299778; PMCID: PMC4649512.
  • Koochekpour S, Marlowe T, Singh KK, Attwood K, Chandra D. Reduced mitochondrial DNA content associates with poor prognosis of prostate cancer in African American men. PLoS One. 2013;8(9):e74688. doi: 10.1371/journal.pone.0074688. eCollection 2013. PMID: 24086362; PMCID: PMC3781126.
  • Gogada R, Yadav N, Liu J, Tang S, Zhang D, Schneider A, Seshadri A, Sun L, Aldaz CM, Tang DG, Chandra D. Bim, a proapoptotic protein, up-regulated via transcription factor E2F1-dependent mechanism, functions as a prosurvival molecule in cancer. J Biol Chem. 2013 Jan 4;288(1):368-81. doi: 10.1074/jbc.M112.386102. Epub 2012 Nov 14. PMID: 23152504; PPMCID: PMC3537034.
  • Gogada R, Prabhu V, Amadori M, Scott R, Hashmi S, Chandra D. Resveratrol induces p53-independent, X-linked inhibitor of apoptosis protein (XIAP)-mediated Bax protein oligomerization on mitochondria to initiate cytochrome c release and caspase activation. J Biol Chem. 2011 Aug 19;286(33):28749-60. doi: 10.1074/jbc.M110.202440. Epub 2011 Jun 28. PMID: 21712378; PMCID: PMC3190683.
  • Jeter CR, Badeaux M, Choy G, Chandra D, Patrawala L, Liu C, Calhoun-Davis T, Zaehres H, Daley GQ, Tang DG. Functional evidence that the self-renewal gene NANOG regulates human tumor development. Stem Cells. 2009 May;27(5):993-1005. doi: 10.1002/stem.29. PMID: 19415763; PMCID: PMC3327393.
  • Chandra D, Choy G, Tang DG. Cytosolic accumulation of HSP60 during apoptosis with or without apparent mitochondrial release: evidence that its pro-apoptotic or pro-survival functions involve differential interactions with caspase-3. J Biol Chem. 2007 Oct 26;282(43):31289-301. doi: 10.1074/jbc.M702777200. Epub 2007 Sep 6. PMID: 17823127.
  • Chandra D*, Bratton SB, Person MD, Tian Y, Martin AG, Ayres M, Fearnhead HO, Gandhi V, Tang DG*. Intracellular nucleotides act as critical prosurvival factors by binding to cytochrome C and inhibiting apoptosome. Cell. 2006 Jun 30;125(7):1333-46. doi: 10.1016/j.cell.2006.05.026. PMID: 16814719.

*Co-corresponding Authors