Orla Maguire

Flow and Image Cytometry

Research Interests:

Developing imaging flow cytometry assays to investigate transcription factor pathways in a clinically relevant setting and assays to investigate the role of exosomes in tumor progression.

About Orla Maguire


My research interests include the use of the ImageStream imaging flow cytometer (Amnis, part of Cytek Biosciences) to provide researchers and clinicians with useful information in the functional activity of transcription factor pathways in immunophenotypically defined populations, allowing for complementary use of current flow cytometry coupled with imaging cytometry information. The results of this endeavor include original publications in Cytometry, Part A, as well as original publications in Journal of Immunological Methods (JIM), and Methods.
We have also developed a modified Fluorescent In Situ Hybridization technique in suspension (FISH-IS) for the ImageStream. This assay enables the automated analysis of several log-magnitude higher numbers of cells compared to the microscopy-based approaches providing the opportunity to detect aneuploidy in relatively rare cell populations. This is clinically relevant as it may be used to detect minimal residual disease and associated chromosomal defects. This assay was first published as an original manuscript in Cytometry, Part A, and the procedure has also been detailed in the book chapter In “Imaging Flow Cytometry: Methods in Molecular Biology” edited by Natasha Barteneva and Ivan Vorobjev. I was honored to receive the Alex Nakeff Young Investigator’s Award at GLIIFCA 2012 for this work. We continue to assist investigators all over the world who are utilizing this assay in their studies.
I have been invited to speak at several national and international conferences and have given tutorials in imaging flow cytometry for the International Society for the Advancement of Cytometry. My unique expertise in imaging flow cytometry has cemented my reputation as a leader in this field. In 2017 I was invited by Science/AAAS to deliver a webinar on monitoring immune function by imaging flow cytometry.
In the emerging field of microparticle analysis by flow and imaging flow cytometry, we are participating with several investigators in various research projects, one of which was chosen for an oral presentation at the 32nd annual CYTO congress in Boston, MA, 2017 (given by former MS student Philip Galbo). We have published the results of that research in Oncotarget. and have another publication with collaborators investigating exosomes by both flow and image cytometry. We have also established an exosome working group between RPCI and the University of Buffalo to facilitate discussion and standardization of methodology.
I am heavily involved in education here at Roswell Park. I am part of the team that has developed the graduate course RPG564, ‘Principles in Flow and Image Cytometry’, tailored to teaching students everything from the basic hardware components of a flow/imaging cytometer, to understanding experimental design, analysis principles, applications including study of cellular phenotype, cell proliferation, cell death, cellular signaling and functional assays. We teach students how to appropriately apply these theories and strategies in planning and critically evaluating flow and image cytometry studies. This course is open to all graduate students and due to its depth, the Tumor Immunology Ph.D. program has deemed it mandatory for their students. In addition to the graduate course, I am also part of the resource education team that maintains an active education program for all users of the facility and resource personnel. These classes cover every aspect of flow and image cytometry from experimental design to instrument use and analysis.


Roswell Park Comprehensive Cancer Center
  • Supervising Flow Cytometrist
  • Flow and Image Cytometry Shared Resource


Education and Training:

  • 2008 - PhD- Functional Genomics (Biomedical Sciences), Ulster University, Northern Ireland, UK

Professional Memberships:

  • 2011-present - International Society for Advancement of Cytometry (ISAC)
  • 2009-present - American Association for Cancer Research (AACR)

Honors & Awards:

  • Alexander Nakeff Young Investigator Award for invited Oral Presentation given at the 21st Annual Great Lakes International Imaging & Flow Cytometry Association Conference
  • Selected as an ISAC Marylou Ingram Scholar for 2018-2022 term


Full Publications list on PubMed

1. Maguire O, Collins C, O’Loughlin K, Miecznikowski J, Minderman H. Quantifying Nuclear p65 as a Parameter for NF-κB Activation: Correlation Between ImageStream Cytometry, Microscopy and Western Blot. Cytometry Part A. 2011 Jun; 79(6): 461-9; PubMed Central PMCID: PMC3140714
2. Maguire O, Chen GL, Hahn TE, Brix L, McCarthy PL, Wallace PK, Minderman H. Quantifying MHC dextramer-induced NFAT activation in antigen-specific T cells as a functional response parameter. Methods. 2017 Jan 1;112:75-83. Pubmed PMID: 27327144; Pubmed Central PMCID: PMC5821144
3. Shenoy GN, Loyall J, Maguire O, Iyer V, Kelleher RJ Jr, Minderman H, Wallace PK, Odunsi K, Balu-Iyer SV, Bankert RB. Exosomes Associated with Human Ovarian Tumors Harbor a Reversible Checkpoint of T-cell Responses. Cancer Immunol Res. 2018 Feb;6(2):236-247. doi: 10.1158/2326-6066.CIR-17-0113. Epub 2018 Jan 4. PubMed PMID: 29301753; PubMed Central PMCID: PMC5845437.
4. Iyer RV, Maguire O, Kim M, Curtin LI, Sexton S, Fisher DT, Schihl SA, Fetterly G, Menne S, Minderman H. Dose-Dependent Sorafenib-Induced Immunosuppression Is Associated with Aberrant NFAT Activation and Expression of PD-1 in T Cells. Cancers. 2019 May 16;11(5). Pubmed PMID: 31100868; PMCID: PMC6562672
5. Elshoury A, Maguire O, Conway A, Tario J, Soh KT, Ross M, Hahn T, Becker J, Wallace P, McCarthy PL, Minderman H, Chen GL. Short-Term Storage of Mobilized Peripheral Blood Stem Cells in a Closed System Changes the Microenvironment and May Affect the Quantity of CD34+ and CD34+CD38-CD45RA-CD90+ Cells. Transplant Cell Ther. 2023 Feb;29(2):112.e1-112.e9. doi: 10.1016/j.jtct.2022.11.021. Epub 2022 Nov 24. PubMed PMID: 36436782.