Dr. John Subjeck Dr. John Subjeck

John Subjeck

PhD

Special Interests:

Heat shock/stress proteins and their use as anti-cancer vaccines.

About Me

Biography:

Dr. John R. Subjeck joined the staff of Roswell Park Comprehensive Cancer Center (Roswell Park) in 1979, as a Cancer Research Scientist, Division of Radiation Biology, Department of Radiation Medicine. He was appointed Member in the Departments of Cell Stress Biology and Molecular and Cellular Biology in 1999, and was also a Professor of the Molecular & Cellular Biophysics and Biochemistry Roswell Park Graduate Division, University at Buffalo (UB).

Dr. Subjeck earned his doctoral degree in Biophysical Sciences, UB School of Medicine & Biomedical Sciences in 1974. He completed a postdoctoral fellowship in Experimental Pathology at Roswell Park in 1977. From 1977-1979, Dr. Subjeck specialized in Hyperthermia as a Research Affiliate in Medical Physics in the Division of Radiation Biology at Roswell Park. He was certified by the American Board of Radiology in Therapeutic Radiological Physics. He Retired from Roswell Park in 2010 but continued his research project until 2014.


Credentials

Positions

Roswell Park Comprehensive Cancer Center
  • Professor Emeritus of Oncology
  • Cell Stress Biology

Background

Education and Training:

  • PhD - Biophysical Sciences, UB School of Medicine and Biomedical Sciences

Publications

Arnouk H, Zynda ER, Wang X-Y, Hylander BL, Manjili MH, Repasky EA,Subjeck JR, Latif Kazim A. Tumour secreted grp170 chaperones full-length protein substrates and induces an adaptive anti-tumour immune response in vivo. International journal of hyperthermia 2010; 26(4):366-375

Wang XY, Sun X, Chen X, Facciponte J, Repasky EA, Kane J, Subjeck JR.Superior antitumor response induced by large stress protein chaperoned protein antigen compared with peptide antigen. Journal of immunology (Baltimore, Md. : 1950) 2010; 184(11):6309-6319

Wang Y, Wang X-Y, Subjeck JR, Kim HL. Covalent crosslinking of tumor antigens stimulates an antitumor immune response. Vaccine 2010;28(40):6613-6620

Gao P, Sun X, Chen X, Subjeck J, Wang X-Y. Secretion of stress protein grp170 promotes immune-mediated inhibition of murine prostate tumor.Cancer immunology, immunotherapy : CII 2009; 58(8):1319-1328

Yi H, Yu X, Gao P, Wang Y, Baek S-H, Chen X, Kim HL, Subjeck JR, Wang X-Y. Pattern recognition scavenger receptor SRA/CD204 down-regulates Toll-like receptor 4 signaling-dependent CD8 T-cell activation. Blood 2009;113(23):5819-5828

Wang Y, Wang X-Y, Subjeck JR, Shrikant PA, Kim HL. Temsirolimus, an mTOR inhibitor, enhances anti-tumour effects of heat shock protein cancer vaccines.British journal of cancer 2011; 104(4):643-652

Hu F, Qiu X, Subjeck JR, Tirosh B, Yi H, Guo C, Zuo D, Wang H, Yu X, Wang XY. ER stress and its regulator X-box-binding protein-1 enhance polyIC-induced innate immune response in dendritic cells. European journal of immunology 2011; 41(4):1086-1097

Yu X, Yi H, Guo C, Zuo D, Wang Y, Kim HL, Subjeck JR, Wang XY. Pattern Recognition Scavenger Receptor CD204 Attenuates Toll-like Receptor 4-induced NF-{kappa}B Activation by Directly Inhibiting Ubiquitination of Tumor Necrosis Factor (TNF) Receptor-associated Factor 6. Journal of biological chemistry 2011; 286(21):18795-18806

Gao P, Sun X, Chen X, Wang Y, Foster BA, Subjeck J, Fisher PB, Wang XY.Secretable chaperone Grp170 enhances therapeutic activity of a novel tumor suppressor, mda-7/IL-24. Cancer research 2008; 68(10):3890-3898

Wang Y, Wang XY, Subjeck JR, Kim HL. Carbonic anhydrase IX has chaperone-like functions and is an immunoadjuvant. Molecular cancer therapeutics 2008; 7(12):3867-3877

Beachy SH, Kisailus AJ, Repasky EA, Subjeck JR, Wang XY, Kazim AL.Engineering secretable forms of chaperones for immune modulation and vaccine development. Methods (San Diego, Calif.) 2007; 43(3):184-193

Facciponte JG, Wang XY, Subjeck JR. Hsp110 and Grp170, members of the Hsp70 superfamily, bind to scavenger receptor-A and scavenger receptor expressed by endothelial cells-I. European journal of immunology 2007;37(8):2268-2279

Kim HL, Sun X, Subjeck JR, Wang XY. Evaluation of renal cell carcinoma vaccines targeting carbonic anhydrase IX using heat shock protein 110.Cancer immunology, immunotherapy : CII 2007; 56(7):1097-1105

Wang XY, Facciponte J, Chen X, Subjeck JR, Repasky EA. Scavenger receptor-A negatively regulates antitumor immunity. Cancer research 2007;67(10):4996-5002

Repasky EA, Subjeck JR. Heat shock proteins and cancer therapy: the trail grows hotter!. Oncotarget 2011; 2(6):433-434

Qian J, Yi H, Guo C, Yu X, Zuo D, Chen X, Kane III JM, Repasky EA, Subjeck JR, Wang X-Y. CD204 suppresses large heat shock protein-facilitated priming of tumor antigen gp100-specific T cells and chaperone vaccine activity against mouse melanoma. Journal of immunology (Baltimore, Md. : 1950) 2011;187(6):2905-2914

Wang X-Y, Yi H, Yu X, Zuo D, Subjeck JR. Enhancing antigen cross-presentation and T-cell priming by complexing protein antigen to recombinant large heat-shock protein. Methods in molecular biology (Clifton, N.J.) 2011;787:277-287

Yi H, Guo C, Yu X, Gao P, Qian J, Zuo D, Manjili MH, Fisher PB, Subjeck JR, Wang X-Y. Targeting the immunoregulator SRA/CD204 potentiates specific dendritic cell vaccine-induced T-cell response and antitumor immunity. Cancer research 2011; 71(21):6611-6620

Guo C, Yi H, Yu X, Hu F, Zuo D, Subjeck JR, Wang X-Y. Absence of scavenger receptor A promotes dendritic cell-mediated cross-presentation of cell-associated antigen and antitumor immune response. Immunology and cell biology 2012; 90(1):101-108

Yi HF, Zuo DM, Yu XF, Hu FL, Manjili MH, Chen ZL, Subjeck JR, Wang XY.Suppression of antigen-specific CD4+ T cell activation by SRA/CD204 through reducing the immunostimulatory capability of antigen-presenting cell. Journal of molecular medicine (Berlin, Germany) 2012; 90(4):413-426

Zuo D, Yu X, Guo C, Yi H, Chen X, Conrad DH, Guo TL, Chen Z, Fisher PB,Subjeck JR, Wang X-Y. Molecular chaperoning by glucose-regulated protein 170 in the extracellular milieu promotes macrophage-mediated pathogen sensing and innate immunity. FASEB journal 2012; 26(4):1493-1505

Wang XY, Yi HF, Yu XF, Guo CQ, Qian J, Zuo DM, Subjeck J. Pattern recognition receptor SRA/CD204 restricts T cell activation and antitumor immunity. Journal of immunology (Baltimore, Md. : 1950) 2012; 188:52.13