Research Interests:
- Heat shock/stress proteins and their use as anti-cancer vaccines.
Biography
Dr. John R. Subjeck joined the staff of Roswell Park Comprehensive Cancer Center (Roswell Park) in 1979, as a Cancer Research Scientist, Division of Radiation Biology, Department of Radiation Medicine. He was appointed Member in the Departments of Cell Stress Biology and Molecular and Cellular Biology in 1999, and was also a Professor of the Molecular & Cellular Biophysics and Biochemistry Roswell Park Graduate Division, University at Buffalo (UB).
Dr. Subjeck earned his doctoral degree in Biophysical Sciences, UB School of Medicine & Biomedical Sciences in 1974. He completed a postdoctoral fellowship in Experimental Pathology at Roswell Park in 1977. From 1977-1979, Dr. Subjeck specialized in Hyperthermia as a Research Affiliate in Medical Physics in the Division of Radiation Biology at Roswell Park. He was certified by the American Board of Radiology in Therapeutic Radiological Physics. He currently serves as a Professor Emeritus, and although retired, he currently collaborates with colleagues both at Roswell Park and at the Virginia Commonwealth University.
Positions
Roswell Park Comprehensive Cancer Center
- Emeritus Faculty
- Department of Cell Stress Biology
Background
Education and Training
- 1974 - PhD - Biophysical Sciences, UB School of Medicine and Biomedical Sciences
Fellowship
- 1977 - Postdoctoral Fellowship in Experimental Pathology - Roswell Park Comprehensive Cancer Center
Board Certification
- Therapeutic Radiological Physics, American Board of Radiology
Research Overview
Structure and function or the large stress proteins, their interaction with the immune response, and the development of stress protein vaccines using the unique properties of these molecular chaperones.
Publications
Full Publications list on PubMed
- Wang XY and Subjeck JR. The high molecular weight stress proteins: identification, cloning, and utilization in cancer immunotherapy. Int. J Hyperthermia 2013, 29(5):364-75. PMC3845003
- Yu XF, Subjeck JR, Wang XY. Integrating a 'danger' signal into molecular chaperoning to improve vaccination against cancer. Expert Rev Vaccines 2013, Jun;12(6):581-3. PMC3682782
- Zuo D, Subjeck J, Wang XY. Unfolding the role of large heat shock proteins: New insights and therapeutic implications. Front Immunol 2016, Mar 1;7:75. PMC4771732
- Guo C, Subjeck JR, Wang XY. Creation of recombinant chaperone vaccine using large heat shock protein for antigen-targeted cancer immunotherapy. Methods Mol Biol 2018, 1709:345-357. PMC5812279
- Chen M, Qiao G, Hylander BL, Mohammadpour H, Wang XY, Subjeck JR, Singh AK, Repasky EA. Adrenergic stress constrains development of anti-tumor immunity and abscopal responses following local radiation. Nat Commun. 2020, Apr 14;11(1):1821. PMC7156731
- Arnouk H, Zynda ER, Wang X-Y, Hylander BL, Manjili MH, Repasky EA,Subjeck JR, Latif Kazim A. Tumour secreted grp170 chaperones full-length protein substrates and induces an adaptive anti-tumour immune response in vivo. International journal of hyperthermia 2010; 26(4):366-375
- Wang XY, Sun X, Chen X, Facciponte J, Repasky EA, Kane J, Subjeck JR.Superior antitumor response induced by large stress protein chaperoned protein antigen compared with peptide antigen. Journal of immunology (Baltimore, Md. : 1950) 2010; 184(11):6309-6319
- Wang Y, Wang X-Y, Subjeck JR, Kim HL. Covalent crosslinking of tumor antigens stimulates an antitumor immune response. Vaccine 2010;28(40):6613-6620
- Gao P, Sun X, Chen X, Subjeck J, Wang X-Y. Secretion of stress protein grp170 promotes immune-mediated inhibition of murine prostate tumor.Cancer immunology, immunotherapy : CII 2009; 58(8):1319-1328
- Yi H, Yu X, Gao P, Wang Y, Baek S-H, Chen X, Kim HL, Subjeck JR, Wang X-Y. Pattern recognition scavenger receptor SRA/CD204 down-regulates Toll-like receptor 4 signaling-dependent CD8 T-cell activation. Blood 2009;113(23):5819-5828
- Wang Y, Wang X-Y, Subjeck JR, Shrikant PA, Kim HL. Temsirolimus, an mTOR inhibitor, enhances anti-tumour effects of heat shock protein cancer vaccines.British journal of cancer 2011; 104(4):643-652
- Hu F, Qiu X, Subjeck JR, Tirosh B, Yi H, Guo C, Zuo D, Wang H, Yu X, Wang XY. ER stress and its regulator X-box-binding protein-1 enhance polyIC-induced innate immune response in dendritic cells. European journal of immunology 2011; 41(4):1086-1097
- Yu X, Yi H, Guo C, Zuo D, Wang Y, Kim HL, Subjeck JR, Wang XY. Pattern Recognition Scavenger Receptor CD204 Attenuates Toll-like Receptor 4-induced NF-{kappa}B Activation by Directly Inhibiting Ubiquitination of Tumor Necrosis Factor (TNF) Receptor-associated Factor 6. Journal of biological chemistry 2011; 286(21):18795-18806
- Gao P, Sun X, Chen X, Wang Y, Foster BA, Subjeck J, Fisher PB, Wang XY.Secretable chaperone Grp170 enhances therapeutic activity of a novel tumor suppressor, mda-7/IL-24. Cancer research 2008; 68(10):3890-3898
- Wang Y, Wang XY, Subjeck JR, Kim HL. Carbonic anhydrase IX has chaperone-like functions and is an immunoadjuvant. Molecular cancer therapeutics 2008; 7(12):3867-3877
- Beachy SH, Kisailus AJ, Repasky EA, Subjeck JR, Wang XY, Kazim AL. Engineering secretable forms of chaperones for immune modulation and vaccine development. Methods (San Diego, Calif.) 2007; 43(3):184-193
- Facciponte JG, Wang XY, Subjeck JR. Hsp110 and Grp170, members of the Hsp70 superfamily, bind to scavenger receptor-A and scavenger receptor expressed by endothelial cells-I. European journal of immunology 2007;37(8):2268-2279
- Kim HL, Sun X, Subjeck JR, Wang XY. Evaluation of renal cell carcinoma vaccines targeting carbonic anhydrase IX using heat shock protein 110.Cancer immunology, immunotherapy : CII 2007; 56(7):1097-1105
- Wang XY, Facciponte J, Chen X, Subjeck JR, Repasky EA. Scavenger receptor-A negatively regulates antitumor immunity. Cancer research 2007;67(10):4996-5002
- Repasky EA, Subjeck JR. Heat shock proteins and cancer therapy: the trail grows hotter!. Oncotarget 2011; 2(6):433-434
- Qian J, Yi H, Guo C, Yu X, Zuo D, Chen X, Kane III JM, Repasky EA, Subjeck JR, Wang X-Y. CD204 suppresses large heat shock protein-facilitated priming of tumor antigen gp100-specific T cells and chaperone vaccine activity against mouse melanoma. Journal of immunology (Baltimore, Md. : 1950) 2011;187(6):2905-2914
- Wang X-Y, Yi H, Yu X, Zuo D, Subjeck JR. Enhancing antigen cross-presentation and T-cell priming by complexing protein antigen to recombinant large heat-shock protein. Methods in molecular biology (Clifton, N.J.) 2011;787:277-287
- Yi H, Guo C, Yu X, Gao P, Qian J, Zuo D, Manjili MH, Fisher PB, Subjeck JR, Wang X-Y. Targeting the immunoregulator SRA/CD204 potentiates specific dendritic cell vaccine-induced T-cell response and antitumor immunity. Cancer research 2011; 71(21):6611-6620
- Guo C, Yi H, Yu X, Hu F, Zuo D, Subjeck JR, Wang X-Y. Absence of scavenger receptor A promotes dendritic cell-mediated cross-presentation of cell-associated antigen and antitumor immune response. Immunology and cell biology 2012; 90(1):101-108
- Yi HF, Zuo DM, Yu XF, Hu FL, Manjili MH, Chen ZL, Subjeck JR, Wang XY.Suppression of antigen-specific CD4+ T cell activation by SRA/CD204 through reducing the immunostimulatory capability of antigen-presenting cell. Journal of molecular medicine (Berlin, Germany) 2012; 90(4):413-426
- Zuo D, Yu X, Guo C, Yi H, Chen X, Conrad DH, Guo TL, Chen Z, Fisher PB,Subjeck JR, Wang X-Y. Molecular chaperoning by glucose-regulated protein 170 in the extracellular milieu promotes macrophage-mediated pathogen sensing and innate immunity. FASEB journal 2012; 26(4):1493-1505
- Wang XY, Yi HF, Yu XF, Guo CQ, Qian J, Zuo DM, Subjeck J. Pattern recognition receptor SRA/CD204 restricts T cell activation and antitumor immunity. Journal of immunology (Baltimore, Md. : 1950) 2012; 188:52.13