Dr. Hans Minderman joined the team of the Experimental Therapeutics Department at Roswell Park Comprehensive Cancer Center in 1990. He was named the Assistant Director, Flow and Image Cytometry Facility in 2007. He earned his doctoral degree in Medical Biology at the University Hospital Nijmegen, The Netherlands (1994).
He has authored or co-authored more than 90 journal publications, abstracts or book chapters.
The Flow and Image Cytometry Facility provides standard and advanced flow cytometry capabilities for research and clinical investigations at RPCI. It is a licensed reference laboratory for classification of leukemias and lymphomas by immunophenotyping, for evaluating immunologically-based diseases, for monitoring transplant patients, and for evaluating DNA in solid tumors. It is committed to translational research through the development of new tests. Standard and advanced flow and image cytometry services and capabilities also are provided to basic research scientists.
Riddell JR, Wang X-Y, Minderman H, Gollnick SO. Peroxiredoxin 1 stimulates secretion of proinflammatory cytokines by binding to TLR4. Journal of immunology (Baltimore, Md. : 1950) 2010; 184(2):1022-1030
Segal BH, Christman JW, Sporn MB, Chan J, Vinh DC, Holland SM, Romani LR, Gaffen SL, Freeman ML, Minderman H, Wallace PK, Capitano M, Han W, Bushey JJ, Joo M, Bhatti Z, Feminella J, Dennis CG, Vethanayagam RR, Yull FE, Blackwell TS. NADPH oxidase limits innate immune responses in the lungs in mice. PloS one 2010; 5(3):e9631
Brady MT, Miller A, Sait S, Ford LA, Hans M, Wang ES, Lee KP, Baumann H, Wetzler M. Down-regulation of signal transducer and activator of transcription 3 enhances acute myeloid leukemia-derived dendritic cell function. Blood 2010;116(21):3140
Minderman H, Pankewycz O, O'Loughlin K, Feng L, Laftavi M, Wallace P. NF-kB translocation as a quantitative parameter for clinical immunosuppression.Cytometry. Part B, Clinical cytometry 2010; 78B(6):410
Maguire O, Collins C, O'Loughlin K, Miecznikowski J, Minderman H.Quantifying nuclear p65 as a parameter for NF-kappaB activation: Correlation between ImageStream cytometry, microscopy, and Western blot. Cytometry. Part A 2011; 79(6):461-469
Brooks TA, O'Loughlin KL, Minderman H, Bundy BN, Ford LA, Vredenburg MR, Bernacki RJ, Priebe W, Baer MR. The 4'-O-benzylated doxorubicin analog WP744 overcomes resistance mediated by P-glycoprotein, multidrug resistance protein and breast cancer resistance protein in cell lines and acute myeloid leukemia cells. Investigational new drugs 2007; 25(2):115-122
Huang RY, Kowalski D, Minderman H, Gandhi N, Johnson ES. Small ubiquitin-related modifier pathway is a major determinant of doxorubicin cytotoxicity in Saccharomyces cerevisiae. Cancer research 2007; 67(2):765-772
Minderman H, Zhou Y, O'Loughlin KL, Baer MR. Bortezomib activity and in vitro interactions with anthracyclines and cytarabine in acute myeloid leukemia cells are independent of multidrug resistance mechanisms and p53 status.Cancer chemotherapy and pharmacology 2007; 60(2):245-255
Pawarode A, Shukla S, Minderman H, Fricke SM, Pinder EM, O'Loughlin KL, Ambudkar SV, Baer MR. Differential effects of the immunosuppressive agents cyclosporin A, tacrolimus and sirolimus on drug transport by multidrug resistance proteins. Cancer chemotherapy and pharmacology 2007;60(2):179-188
Melchjorsen J, Ostergaard L, Minderman H, Hedley DW, Ellermann-Eriksen S, Paludan SR, Chow S, O'loughlin KL, Sogaard OS, Risor MW, Tolstrup M.Tenofovir selectively regulates production of inflammatory cytokines and shifts the IL-12/IL-10 balance in human primary cells. Journal of acquired immune deficiency syndromes (1999) 2011; 57(4):265-275
Brady MT, Sait SNJ, Minderman H, Lee KP, Wetzler M. Knock-Down of Signal Transducer and Activator of Transcription 3 Enhances Acute Myeloid Leukemia-Derived Dendritic Cell Function. Blood 2009; 114(22):781
Collins CC, O'Loughlin ML, Wallace PK, George T, Miecznikowski JC,Minderman H. Quantitative assessment of NF-kappa B activation in heterogeneous cell populations. Proceedings of the American Association for Cancer Research Annual Meeting 2009; 50:667
Huang R-Y, Kowalski D, Minderman H, Gandhi N, Johnson ES. SUMO pathway is a major determinant of doxorubicin cytotoxicity in yeast. Proceedings of the American Association for Cancer Research Annual Meeting 2007; 48:171
O'Loughlin KL, Minderman H, Ford LA, Baer MR. Amonalide L-malate bypasses multidrug resistance proteins in secondary acute myelloid leukemia.Blood 2007; 110(11 Part 1):Abstract #2380
Pankewycz OG, Minderman H, Wallace P, Feng L, Laftavi MR. A Novel Method To Assess the Level of In Vivo Immunosuppression in Transplant Patients Using Amnis ImageStream Technology. American journal of transplantation2009; 9(Suppl. 2):658
Mace TA, Zhong L, Kilpatrick C, Zynda E, Lee CT, Capitano M, Minderman H, Repasky EA. Differentiation of CD8+ T cells into effector cells is enhanced by physiological range hyperthermia. Journal of leukocyte biology 2011;90(5):951-962
Tornatore K, Minderman H, McQuire O, O'Louglin K, Shan G, Wilding G, Venuto R. Nuclear Localization of Nuclear Factor of Activated T-Cells as a Pharmacodynamic Marker of Tacrolimus Immunosuppression in Renal Transplant Recipients. American journal of transplantation 2012; 12(Suppl. 3):285
Minderman H, Arcadi JC, Wierzbicki A, Wang ES, Sait SNJ, Block AW, Liang L, George T, Wallace PK. Detection of Numerical Chromosome Aberrations in Rare Acute Myeloid Leukemia (AML) Cells. Haematologica 2009; 94(Suppl. 2):0037
Minderman H, O'Loughlin K, Collins C, Venkatachalam V, George T, Wallace PK. Quantitative measurement of nuclear translocation of NF-kB and Stat-3 as parameter of response to targeted treatments in immunophenotypically defined cells in acute myeloid leukemia. Haematologica 2009; 94(Suppl. 2):0751
Tornatore KM, DiFrancesco R, Sudchada P, Gundroo A, Minderman H, O'Loughlin K, Wilding G, Danison R, Venuto RC. Assessment of P-glycoprotein Function in Peripheral Mononuclear Cells in African American and Caucasian Renal Transplant Recipients During Chronic Cyclosporine Therapy. Clinical pharmacology and therapeutics 2009; 85(Suppl. 1):S75
Minderman H, Humphrey K, Arcadi JK, Wierzbicki A, Maguire O, Wang ES, Block AW, Sait SN, George TC, Wallace PK. Image cytometry-based detection of aneuploidy by fluorescence in situ hybridization in suspension. Cytometry. Part A 2012; 81A(9):776-784