Dr. Barbara W. Henderson earned her doctoral degree in Zoology at the University of Graz, Austria, in 1966 and joined the staff of Roswell Park Comprehensive Cancer Center (RPCI) in 1970, as a Cancer Research Scientist. Dr. Henderson was the Program Leader, Biophysical Therapies Program, and the first Chair of the Department of Cell Stress Biology. She retired from these positions in 2007. Dr. Henderson, Professor of Oncology, currently is the Director of the Photodynamic Therapy Center at RPCI.
Dr. Henderson's laboratory research has provided numerous seminal insights into the mechanisms and strategies for optimization of photodynamic therapy (PDT); oxygen and light dependency of PDT; the effect of PDT on the host immune response against tumor; and determinants of photodynamic tumor destruction.
Dr. Henderson is a member of the American Association for Cancer Research, American Society of Photobiology, International Photodynamic Association, and the Radiation Research Society. She has for many years served as Associate Editor of the journal Photochemistry and Photobiology. She is a past member of the NCI Radiation Study Section and Subcommittee D (Clinical Studies) of the NCI Initial Review Group. She is a member of numerous committees at RPCI and has authored or co-authored more than 80 journal publications, book chapters and abstracts.
Description of Research
Photodynamic Therapy: Basic and Translational Research
The objective of this program is to devise and implement more effective approaches to Photodynamic Therapy (PDT). PDT involves the administration of a photodynamically-active drug (photosensitizer) or pro-drug followed by drug-activating visible light. This therapy has been successfully applied to both neoplastic and non-neoplastic diseases. Dr. Henderson is the Principal Investigator of a Program Project Grant (2009-2014) entitled “PDT: Mechanisms and Strategies for Optimization”.
This research program takes place in the multidisciplinary, highly interactive environments of the Photodynamic Therapy Center and the Biophysical Therapies Program. As such, numerous lines of attack are being taken to improve both the efficacy and selectivity of PDT, including (i) the rational design, synthesis and testing of new photosensitizers [directed by Dr. Ravindra Pandey, (ii) the design and application of optimal therapeutic regimens, e.g., drug dosage and schedule based on phamacokinetic/pharmacodynamic studies [directed by Dr. David Bellnier, (iii) the elucidation and exploitation of PDT-induced immune responses [directed by Dr. Sandra Gollnick and (iv) the study of molecular pathways of PDT action [directed by Dr. Heinz Baumann.] The program has clinical research components in the areas of lung cancer and head & neck cancer.
Sunar U, Rohrbach D, Rigual N, Tracy E, Keymel K, Cooper MT, Baumann H,Henderson BH. Monitoring photobleaching and hemodynamic responses to HPPH-mediated photodynamic therapy of head and neck cancer: a case report. Optics express 2010; 18(14):14969-14978
Henderson BH. Monitoring photobleaching and hemodynamic responses to HPPH-mediated photodynamic therapy of head and neck cancer: a case report. Optics express 2010; 18(14):14969-14978
Rigual NR, Thankappan K, Cooper M, Sullivan MA, Dougherty T, Popat SR, Loree TR, Biel MA, Henderson B. Photodynamic therapy for head and neck dysplasia and cancer. Archives of otolaryngology--head and neck surgery2009; 135(8):784-788
Zheng X, Henderson BW, Bellnier DA, Jackson J, Batt C, Missert JR, Baumann H, Tracy E, Chen Y, Pandey SK, Morgan J, Pandey RK. Conjugation of 2-(1'-hexyloxyethyl)-2-devinylpyropheophorbide-a (HPPH) to carbohydrates changes its subcellular distribution and enhances photodynamic activity in vivo.Journal of medicinal chemistry 2009; 52(14):4306-4318
Becker TL, Paquette AD, Keymel KR, Henderson BW, Sunar U. Monitoring blood flow responses during topical ALA-PDT. Biomedical optics express 2010;2(1):123-130
Seshadri M, Bellnier DA, Vaughan LA, Spernyak JA, Mazurchuk R, Foster TH,Henderson BW. Light delivery over extended time periods enhances the effectiveness of photodynamic therapy. Clinical cancer research 2008;14(9):2796-2805
Henderson BW, Daroqui C, Tracy E, Vaughan LA, Loewen GM, Cooper MT, Baumann H. Cross-linking of signal transducer and activator of transcription 3--a molecular marker for the photodynamic reaction in cells and tumors. Clinical cancer research 2007; 13(11):3156-3163
Kousis PC, Henderson BW, Maier PG, Gollnick SO. Photodynamic therapy enhancement of antitumor immunity is regulated by neutrophils. Cancer research 2007; 67(21):10501-10510
Pandey SK, Dougherty TJ, Henderson BW, Oseroff AR, Bellnier DA, Shibata M, Liu TH, Missert JR, Morgan J, Zheng X, Pandey RK. Purpurinimide carbohydrate conjugates: effect of the position of the carbohydrate moiety in photosensitizing efficacy. Molecular pharmaceutics 2007; 4(3):448-464
Wei LH, Baumann H, Tracy E, Wang Y, Hutson A, Rose-John S, Henderson BW. Interleukin-6 trans signalling enhances photodynamic therapy by modulating cell cycling. British journal of cancer 2007; 97(11):1513-1522
Nava HR, Allamaneni SS, Dougherty TJ, Cooper MT, Tan W, Wilding G,Henderson BW. Photodynamic therapy (PDT) using HPPH for the treatment of precancerous lesions associated with barrett's esophagus. Lasers in surgery and medicine 2011; 43(7):705-712
Seshadri M, Spernyak JA, Mazurchuk R, Vaughan LA, Bellnier DA, Henderson BW. Fluence rate effects on tumor oxygenation during photodynamic therapy for cancer: A BOLD (fMRI) experience. Proceedings of the American Association for Cancer Research Annual Meeting 2007; 48:1292-1293
Tracy EC, Bowman MJ, Pandey RK, Henderson BW, Baumann H. Cell-type selective phototoxicity achieved with chlorophyll-a derived photosensitizers in a co-culture system of primary human tumor and normal lung cells.Photochemistry and photobiology 2011; 87(6):1405-1418
Rohrbach DJ, Rigual N, Tracy E, Kowalczewski A, Keymel KL, Cooper MT, Mo W, Baumann H, Henderson BW, Sunar U. Interlesion differences in the local photodynamic therapy response of oral cavity lesions assessed by diffuse optical spectroscopies. Biomedical optics express 2012; 3(9):2142-2153