Translational Imaging Equipment

The Resource's imaging instrumentation includes:

  • A 4.7 Tesla small animal MR scanner incorporating Bruker AVANCE™ electronics and the ParaVision® version 4.0 imaging platform (Bruker Biospin). The system contains a S150 Accustar actively-shield gradient set suitable for imaging larger rodents or whole-body mice, as well as a Bruker BG6 (60cm) gradient set insert capable of generating 950 mT/m magnetic gradients to achieve <75 micron resolution. Both gradient sets are capable of acquiring echo-planer, sub-second imaging. Several radiofrequency whole-body (35 to 112mm ID) and surface coils (7 to 20 mm) are available for imaging both hydrogen (1H) and fluorine nuclei (19F) in rodents.
  • An MR compatible animal monitoring & gating system (Model 1025, SAII Instruments, Stony Brook, NY), which allows for the monitoring of body temperature, heart rate, and respiratory rate of anesthetized animals, as well as providing warm air heating for anesthetized animals undergoing MR imaging.
  • A combined bioluminescence/fluorescence tomographic optical imaging system (IVIS Spectrum, Perkin-Elmer). Acquired in 2013, this system allows for a) efficient and highly sensitive bioluminescent imaging, b) near-infrared fluorescent imaging with spectral unmixing capabilities, c) quantitative fluorescent imaging via transillumination techniques and d) 3D optical tomography for localization of emission signal and coregistration to magnetic resonance imaging data. The Spectrum system features a thermoelectrically-cooled charge-coupled device (CCD) camera (nominal operating temp -90°C, CCD sensor size: 6.76 cm2, pixels: 2048 x 2048) with four magnification settings capable of imaging fields of view ranging from 3.9 cm x 3.9 cm (20 micron resolution) to 23cm x 23 cm, allowing for the simultaneous imaging of 5 mice. For fluorescence imaging, excitation light is provided by a 150W quartz tungsten halogen lamp situated behind a filter wheel comprised of 10 narrow band excitation filters (430-745 nm, 30 nm bandpass). Trans-illumination can be spatially localized or rasterized by means of x-y translation of the light path. A 24-position filter wheel featuring 18 emission filters (500-840nm, 20 nm band pass) is situated in front of the camera for spectral demixing capabilities. A laser galvanometer provides surface topography and alignment capabilities.
  • A bioluminescence imaging system (IVIS-50, Perkin-Elmer) for real-time in vitro/in vivo imaging of luciferase expression. The system is capable of imaging three mice simultaneously and has been recently upgraded to include a -90°C Andor CCD camera for reduced dark-charge signal and enhanced sensitivity.
  • A small animal Ultrasound/Photoacoustic imaging system (VevoLAZR,VisualSonics Inc) system was successfully installed in 2012. The system is based on linear array technology that enables collection of photoacoustic and ultrasound imaging datasets on the same plane, thereby enabling co-registration of structural and functional information on the tissues of interest. The VevoLAZR system provides an integrated, compact and safe platform for in vivo imaging of morphology and microvascular dynamics of tumors in 2D and 3D. The laser source for the system is an optical parametric oscillator (OPO) pumped by Nd:YAG with a tunable range of 680-950 nm. Combined use of PA mode imaging along with B-mode imaging gives both structural information on tumor morphology and functional information on tumor blood flow and oxygenation.
  • A fully digital, cabinet X-ray radiography system (Faxitron MX-20) for animal imaging with adjustable radiation dose for desired contrast. The system provides multiple magnification levels allowing for a maximum field of view of 100x150 mm or maximum resolution of 50 lp/mm.
  • A fiber-optic based, minimally invasive system for measuring tissue pO2 and Doppler blood flow (OxyFlo/OxyLight, Oxford Optronix).

Software includes:

  • Analyze 7.0 (AnalyzeDirect): Versatile tool chest of medical image processing modules including: volumetric and intensity quantification of 2D/3D/4D image datasets, three-dimensional volume rendering, and object/cell counting.
  • Amira 5.3 (Visage Imaging, Inc.): 2D/3D quantification, visualization & animation software.
  • Matlab (MathWorks): with image acquisition and processing toolboxes for programming customized image processing routines. A suite of over 50 custom-developed functions are available for efficient analysis of image-based datasets.