The SKY/FISH facility was established in January 2000, through the support of the J.H. Cummings Foundation, to provide comprehensive cancer cytogenetics services. Services include conventional chromosome analysis such as G-banding (Human tissue only) and fragile site analysis as well as more specialized techniques such as Spectral karyotyping (SKY) and fluorescent in situ hybridization (FISH). These techniques are useful in identifying chromosomal abnormalities on a global scale or more specific gene level mutations in a variety of species such as human, mouse and rat.
The following are SKY/FISH detailed services:
- Spectral karyotyping (SKY) to identify chromosomes or chromosomal segments that are structurally and numerically altered in cells.
- G-Banding analysis (Human samples only) to identify chromosome number as well as chromosome aberrations including translocations, insertions, deletions or duplications in cells.
- Fluorescence in-situ hybridization (FISH) analysis using up to three-fluorescent colors to detect and localize genes, gene sets, fusion genes, telomere-specific sequences, centromere-specific sequences and specific chromosomal regions.
- Combined analysis by SKY and FISH to determine whether rearrangement, deletion or amplification occurs in genes encoding tumor suppressors, cell cycle factors, factors involved in immunological responses, DNA-damage responses, drug resistance, transgene expression, etc.
- Quantification of gene copy number in transgene research material. This method is also useful for investigators who want to identify transgene locations.
- Chromosome aberration and fragile site analysis
- Planning of inter-disciplinary projects utilizing SKY/FISH technology, data interpretation and technical education and guidance for standard cytogenetic protocols.
Investigators interested in the use of the facility may contact Mr. Leighton Stein at 716-845-3213 or at Leighton.Stein@roswellpark.org to discuss scheduling and procedures.