Flow Cytometric Based Cytotoxicity Assays

This cell-mediated cytotoxicity assay is an alternative to the standard chrominum-51 (51Cr) release assays. Target cells are labeled with the cell tracking dye, CFSE, and incubated with effector cells. 7-AAD, which binds to DNA and can only enter membrane-compromised cells, is used as an indicator of cell death.

Please contact Dr. Junko Matsuzaki at (716) 845-8459 or Junko.Matsuzaki@roswellpark.org for a project specific cost estimate.