Primers We Provide

T7/T3/SP6/BGH/CMV Sequence Base # MW TM
T3 ATT AAC CCT CAC TAA AG 17 5122 40
T3cs A ATT AAC CCT CAC TAA AGG G 20 6092 48
T7 AAT ACG ACT CAC TAT AG 17 5161 40
SP6 ATT TAG GTG ACA CTA TAG AA 20 6161 44
BGH reverse TAG AAG GCA CAG TCG AGG 18 5592 50
CMV forward CGC AAA TGG GCG GTA GGC GTG 21 6553 60
Bluescript primers Sequence Base # MW TM
KS CTC GAG GTC GAC GGT ATC GA 20 6152 56
SK CGC TCT AGA ACT AGT GGA TC 20 6113 52
BL-REV GGA AAC AGC TAT GAC CAT G 19 5842 49
pUC/M13 primers Sequence Base # MW TM
M13-48R AGC GGA TAA CAA TTT CAC ACA GGA 24 7375 54
M13-21 T GTA AAA CGA CGG CCA GT 18 5529 48
M13-20 GTA AAA CGA CGG CCA GT 17 5224 47
M13-47 CGC CAG GGT TTT CCC AGT CAC GAC 24 7285 63
M13-reverse CAG GAA ACA GCT ATG ACC 18 5502 50
pGEX primers Sequence Base # MW TM
pGEX 5’ GGG CTG GCA AGC CAC GTT TGG TG 23 7128 62
pGEX 3’ CCG GGA GCT GCA TGT GTC AGA GG 23 7137 62
pET primers Sequence Base # MW TM
T7 promoter TAA TAC GAC TCA CTA TAG GG 20 6122 48
T7 terminator GCT AGT TAT TGC TCA GCG G 19 5830 51
pGL/pCAT primers Sequence Base # MW TM
GL primer 1 TGT ATC TTA TGG TAC TGT AAC TG 23 7056 50
GL primer 2 CTT TAT GTT TTT GGC GTC TTC CA 23 6975 52
RV primer 3 CTA GCA AAA TAG GCT GTC CC 20 6083 52
RV primer 4 GAC GAT AGT CAT GCC CCG CG 20 6098 58