Sanger Sequencing

General Information and Pricing - General information about the sequencing lab, such as pricing, services we provide, where we are located and hours of operation..


Online ordering -  With our web-based ordering system, you will be able to submit your orders electronically and retrieve your sequence data when completed. This online resource is now available at https://biopolymer.roswellpark.org.

Please see .pdf attached below with ordering instructions.

Sample Requirements and Sequencing Basics - Overview the basics of automated fluorescent sequencing and how it works. Also provided is a table of types of DNA we can sequence and required concentrations, our recommendations for template preparation, and primer design.

Interpreting your chromatograms - Advice on how to evaluate your chromatogram and sequence data quality. 

Troubleshooting your data - Common sequencing dilemmas, reasons why you might encounter them, and what you can do to try and solve these problems.

Primers we provide - Our facility provides numerous primers, free of charge, to be used when sequencing many of the more common vectors. A table here lists their names, sequences and molecular weights.

ABI viewers - In order to view the ABI color files we now provide for downloading from our server, you will need specific software to open and work with these files. In this section, we provide links to websites offering freeware for downloading to your computer.

Useful links - Useful websites. 

Contact: 

Khin Marlar (716-845-8032)

Roswell Park Cancer Institute                                                                                                                                             Genomics Shared Resource                                                                                                                                                     DNA Sequencing Laboratory                                                                                                                                               Center for Genetics and Pharmacology, L1-140                                                                                                                   Elm and Carlton Sts.                                                                                                                                                             Buffalo, NY 14263

 

The DNA sequencing services provided by RPCI’s Genomic Shared Resource are intended solely for research purposes.  These DNA sequencing services are not intended or certified by the New York State Department of Health for use in clinical diagnostic testing or treatment, including but not limited to patient education.  
 
Roswell Park Cancer Institute shall have no liability for claims by, or damages of any kind whatsoever to, a user of these DNA sequencing services for a decision or action taken in reliance on the information provided in the DNA sequencing.  Such damages include, without limitation, direct, indirect, special, incidental or consequential damages. 

 


For questions or problems with this website, contact michael.logar@roswellpark.org.