The GSR prepares RNA and DNA from a variety of sample types, using several extraction protocols depending on the sample size and downstream application.
DNA (cells, tissues, FFPE): Gentra Puregene, Qiagen DNeasy
RNA (cells, tissues, FFPE): TRIzol, Qiagen RNeasy, PAXgene
miRNA enriched: Qiagen miRNeasy
Nucleic Acid Quality control
NanoDrop: A260, A230, A280
Qubit and SpectraMax fluorescent plate reader: picogreen, ribogreen quantitations
Bioanalyzer 2100: RNA and DNA chips
Samples - A minimum of 1M cells or 25mg of tissue is required for RNA/DNA preparation. All samples must be fresh or snap frozen in liquid nitrogen and stored at -80°C. Blood: 200ul minimum for automated DNA preparation.