The Genomics Shared Resource currently houses two ABI 7900HT Sequence Detection Systems for quantitative Real Time-PCR or end-point detection assays. The instruments can be scheduled for use by RPCI investigators, or full services performed for:
- TaqMan gene expression (individual probes and fluidics cards)
- TaqMan miRNA expression
- TaqMan SNP genotyping
- Exiqon miRNA RT-PCR panels and individual primers
- Copy Number Validation (SYBR green)
Those interested in using the ABI 7900HT Sequence Detection System must first speak with Sean Glenn, ext. 4012. He will arrange a time for you to be trained on the system, and to set up an account so that you may schedule equipment time. Once an account has been established all 7900HT scheduling is done via Resource Scheduling in Outlook Express Public Folders.
The investigator is responsible for purchasing kits and consumables. The Resource will maintain the instruments, provide training, and make primer design and analysis software available. Primer Express v2.0 software is available through the facility, call for more information. There is a $27.00 charge for each plate run (up to 3 hours).
Please note the following criteria for the ABI 7900HT Systems:
- The only sample containers that can be used are Semi- or Half-Skirted well plates with flat-tops. Users can not use strip-tubes, rounded-caps, or unskirted plates. Users will also need to purchase flat-top, optical caps or 0.2 mm thermal microseal film for their plates. All other plates and caps will not work with these machines. These items may be purchased through any company (ex: VWR or Krackeler.)
- Microsoft Windows platform is used instead of Macintosh.