The Genomics Shared Resource (GSR) offers sample-to-data services, with an expert technical staff performing all aspects of sample preparation, QC, assay design and analysis. The mission of the GSR is to provide state-of-the-art instrumentation and expertise that enables its users to acquire and analyze genomic data sets across basic, translational, clinical and population studies. Our long-standing history and contribution to the Human Genome Program through clone generation, high-throughput mapping, array technology development, and distribution of these resources worldwide provides a track record documenting our expertise that can be extended to the outside community. The GSR offers investigators a full spectrum of services, including Next Generation sequencing (NGS), genotyping (targeted and global), methylation (targeted and global), copy number and expression analysis (gene and miRNA), and Sanger sequencing. The facility houses an Illumina iScan system, two Applied Biosystems QuantStudio 6 Real-Time PCR System, two Applied Biosystems 3500 Genetic Analyzers, Caliper Sciclone NGS workstation, 10x Genomics Chromium system and Nanostring nCounter systems, as well as Illumina (NovaSeq, NextSeq and MiSeq) sequencers. The GSR provides a full complement of NGS services, including Whole Genome Sequencing (WGS), Whole Exome-seq (WES), RNA-seq, WGBis-seq (Methyl-seq), ChIP-Seq, targeted, and small RNA-seq. Single-cell RNA-seq and DNA applications can also be performed within the core using the 10x Genomics Chromium, allowing for resolution of genomic content down to a single cell. The GSR also provides methylation analysis of intact or compromised DNA samples using the Illumina Infinium MethylationEPIC BeadChip (global) as well as full-service gene expression analysis and SNP/CNV (300K-4.5M SNPs) genotyping using Illumina BeadChip technology. The core provides many levels of customizable validation technologies to fit any sized project, including custom Illumina BeadChips, qPCR, nCounter digital detection assays, and Sanger sequencing. The GSR is also a repository for the RP11 and RP23 BAC genomic clone libraries which are available for clone selection, characterization, FISH mapping, and distribution. The GSR is a state-of-the-art facility which utilizes a variety of platforms to accommodate any size request and monitors all processes using a LIMS that includes on-line requests, sample submission, workflow and sample tracking to ensure quality reproducible data generated efficiently.