The Gene Targeting and Transgenic Resource enables investigators to create genetically modified mice by several approaches for the systematic dissection of the genetic, molecular, cellular and physiological mechanisms underlying complex biological processes. The Resource also provides mouse reproductive technology services to investigators to bank and share their valuable mouse strains, to clean their strains of unwanted pathogens, and to rapidly expand their mouse colonies.
In addition, the GeTT has recently expanded to provide Mouse Colony Management Services to CCSG investigators. This includes breeding and genotyping of mouse colonies to deliver the multigenic mouse cohorts needed for research.
GeTT provides fee-for-service, first-come-first-serve pronuclear injection, gene targeting, blastocyst injection, cryopreservation and rederivation to Roswell Park Comprehensive Cancer Center, The State University of New York at Buffalo (UB), and other academic investigators at a reasonable price with fast turnaround. Our resource is the sole local provider of these services for the Buffalo and Western New York biomedical research community. We have an extensive record of high efficiency and success in these services, including the creation of conditional knockout and transgenic mice.
The facility has roots in the Departments of Molecular and Cellular Biology, which go back to the early 1990s. In June of 1999, the GeTT was made a formal shared resource supported by the Roswell Park Comprehensive Cancer Center and the National Cancer Institute’s CCSG Core Grant, serving the current user base.
The importance of this resource can be seen by the increased demand from within the CCSG programs to develop genetically modified mice. Since it was established, 38 program members have utilized the resource and have generated over 700 different transgenic mouse models and over 200 knockout models. Since it is a unique regional resource, investigators at UB have asked to use its services, and, in 2004, a contract for service was established, and this contract has been extended annually. It is anticipated that utilization of the resource will increase substantially in the next project period due to increased demand from program members, new recruits, and the implementation of new services including use of the powerful genome-editing tool platform. Increased demand is also expected due to the need for validation of high-throughput clinical findings from the success of Roswell Park’s Center for Personalized Medicine and clinical-based research. Cancer research based on human genomics stimulates the need for creation of genetically modified mice and underscores the importance of increasing efficiency in developing mouse models of human cancers.
The GeTT is one of several animal-based shared resources currently at Roswell Park. The other three animal resources are: the Laboratory Animal Shared Resource (LASR), the Mouse Tumor Model Shared Resource (MTM), and the Translational Imaging Shared Resource (TISR). The GeTT works in collaboration with all of the other animal-based resources in a symbiotic fashion. GeTT staff create the genetically-modified mice, while LASR maintains the mouse colonies; MTM breeds the colonies and provides experimental mouse services, and TISR provides animal imaging of experimental animals.