Subcloning

In certain instances, ES cells are found to be aneuploid (greater than or less than 40 chromosomes). Aneuploid ES cells may generate chimeras; however they will never be transmitted through the germline. If you have an aneuploid ES cell clone, it may be possible to rescue that clone by plating it at a low density and picking subclones that would be diploid, and thus transmit through the germline.

Please contact Aimee Stablewski at (716) 845-5843 or Aimee.Stablewski@roswellpark.org for a project specific cost estimate.