The Gene Targeting and Transgenic Shared will prepare new mouse ES cell lines from blastocysts or mice provided by investigators. Standard methods employing serum containing medium and MEK1 inhibitor are used. The success rate of this procedure is high as long as blastocysts can be obtained from the mouse strain in question. Mouse ES cells provide a endless supply of cells for in vitro studies because ES cells do not undergo senescence and cease division as do other cell types (e.g., fibroblasts).
In addition, it is possible to differentiate ES cells into different lineages to query gene function in cell culture systems instead of or in parallel to in vivo studies. The procedure requires intensive cell culture over an extended period of time by shared resource personnel. Advance notice should be given, preferably when the blastocyst-donors-to-be are born. The ideal age for of in-house blastocyst donors for superovulation response is 24-28 days.
Please contact Aimee Stablewski at (716) 845-5843 or Aimee.Stablewski@roswellpark.org for a project specific cost estimate.