Imagestream Services

The ImageStreamX Mark II Imaging Flow Cytometers combine the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and morphological insights of microscopy.

Our operations run the spectrum from independent use of our ImageStreams by trained researchers, to complete service projects in which cell preparation, staining, data acquisition and analysis are carried out entirely by our resource staff – and everything in between.

The FICSR houses two nearly identical ImageStreamX Mark II instruments (see specific instrument pages for differences). These instruments are accessible 24/7 to all trained investigators for an hourly based usage fee. Training consists of attending the free class Introduction to Flow Cytometry, which provides general information about the use of our facility as well as a basic overview of the flow cytometry technology.

Once this class has been completed, three free one-on-one training sessions to become proficient specifically on the ImageStream instruments is available by appointment only with our staff.

Our goal is to train investigators to work in the facility independently; however, fee-based continued assistance is also an option.

The facility also houses four dedicated analysis workstations with the proprietary IDEAS software (for details see Analysis Software) that are available free of charge and (as of yet) there is no need for reservations.

Please contact Hans Minderman (716) 845-1162 or Orla Maguire (716) 845-5890 for further information.

If you are using the ImageStream imaging flow cytometer, please acknowledge the Shared Instrument Grant that funded the acquisition on this instrument as follows:

“The ImageStream-MKII instrument was funded through the NIH Shared Instrument Grant S10OD18048”

*For information on Upcoming Classes 

Technical Specifications

  • 12 channel, 2 camera instrument
  • Up to 10 fluorescent parameters with two brightfield images
  • Side scatter available in either channel 6 or channel 12 (from dedicated 785nm scatter laser)
  • Auto sampler option (96-well plate format)
  • PC Windows INSPIRE® version 200.1.388

Excitation Sources

  • 405nm: 0-120mW
  • 488nm:0-200mW
  • 561nm: 0-200mW
  • 592nm: 0-300mW
  • 642nm: 0-150mW

Optics

  • 20x, 40x, 60x magnification
  • Extended Depth of Field (EDF) option (16 µ)

Emission Detected

Camera 1: 488nm, 561nm,and 592nm excitation

  • Ch1: 420-480 nm
  • Ch2: 480-560 nm
  • Ch3: 560-595 nm
  • Ch4: 595-642 nm
  • Ch5: 642-745 nm
  • Ch6: 740-800 nm (scatter)

Camera 2: 405nm,and 642nm excitation

  • Ch7: 420-505 nm
  • Ch8: 505-570 nm
  • ◦Ch9: 570-595 nm
  • Ch10: 595-660 nm
  • Ch11: 660-740 nm
  • Ch12: 740-800 nm

ISX205: Auto sampler option (96-well plate format)

 

Technical Specifications

  • 12 channel, 2 camera instrument
  • Up to 10 fluorescent parameters with two brightfield images
  • Side scatter available in either channel 6 or channel 12 (from dedicated 785nm scatter laser)
  • PC Windows INSPIRE® version 200.1.388

Excitation Sources

  • 405nm: 0-120mW
  • 488nm:0-200mW
  • 561nm: 0-200mW
  • 642nm: 0-150mW

Optics

  • 20x, 40x, 60x magnification

Emission Detected

Camera 1:488nm and 561nm excitation

  • Ch1: 420-480 nm
  • Ch2: 480-560 nm
  • Ch3: 560-595 nm
  • Ch4: 595-642 nm
  • Ch5: 642-745 nm
  • Ch6: 745-800 nm

Camera 2:405 nm and 642nm excitation

  • Ch7: 420-505 nm
  • Ch8: 505-570 nm
  • ◦Ch9: 570-595 nm
  • Ch10: 595-642 nm
  • Ch11: 642-745 nm
  • Ch12: 745-800 nm