Education

The Department of Flow and Image Cytometry teaches various free courses to educate the Roswell and Buffalo community on the techniques employed in our facility. Courses typically take about 3 hours and have a lecture presentation as well as a lab demo part. For information on the dates and location of our classes, please consult the sign-up sheets in the hallway next to the Grossberg library (CCC Rm 314) or contact us at (716) 845-8471.

For beginners in Flow Cytometry and those new to our facility, we request that you take the Introduction to Flow Cytometry course before using the instruments outside regular business hours when no supervision is available. One-on-one training on the equipment is freely available to everyone upon prearrangement with the core staff. It is highly recommended that after taking the Introductory Course you schedule a one-on-one session with a member of our staff when you are ready to acquire and analyze your data. Please contact us at ext. 8471.

On this website, you will find links to various resources that provide useful background information and tutorials. If you are new to flow cytometry, you will find it particularly useful to view the 12-minute Invitrogen tutorial “How a flow cytometer works” before you embark on any experiments.

For basic flow cytometry information and tutorials see our Educational links 
For data analysis software information and tutorials see our Software links. 
See course description links below.  To send an email to sign up for a class, click here.

Upcoming Courses

Offered on a monthly basis, this course is mandatory for those who want to use the flow cytometers on their own. The course covers facility logistics, flow cytometry basics, data analysis basics, and the use of proper controls. A simple instrument set-up with compensation is demonstrated on a DiVa-based instrument (LSRII or Fortessa).

Next Class:
Monday August 27, 2018
1pm-3:30pm
Grossberg Classroom CCC314
SIGN UP

 

The introduction to ImageStream cytometry course is a 3-hour course that is divided into two parts. During the first part, the basics on how the ImageStream works and its similarity and differences with conventional flow cytometry will be presented. Various examples of the most common ImageStream applications will be shown and tips and tricks and potential pitfalls for experimental set up will be discussed. The second part is a hands on / interactive instruction (in the RSC computer class room) on using the IDEAS analysis software. For this part the basic features of the IDEAS software are explained by analyzing actual data sets. As a follow-up to this introductory course, one-on-one sessions should be scheduled with our operators for instruction on instrument set up and data acquisition.

Next Class: 
Thursday October 18, 2018
1:00pm-3:00pm
Grossberg Classroom CCC314
SIGN UP

 

This course introduces the basic concepts of fluorescent microscopy, as well as the understanding of confocal microscopy and its applications. Specific focus is on how to use our Leica SP2 confocal microscope.

Next Class: 
Thursday September 27, 2018
1:00pm-3:00pm
Grossberg Classroom CCC314
SIGN UP

 

For those who have learned flow cytometry basics, this course gives intermediate-level users the opportunity to learn the BD DiVa software application more in-depth. The focus is primarily on experiment set-up and acquisition with compensation.

Next Class:
September, 2018
10am-12pm
Grossberg Classroom CCC314
SIGN UP

 

This course outlines various methods for DNA ploidy and cell cycle analysis and their applications. The practical part focuses on cell preparation for DNA staining with propidium iodide, followed by flow cytometry acquisition on the FACS Calibur and ModFit analysis.

Next Class: 
Thursday November 15, 2018
1:00pm-4:00pm
Grossberg Classroom CCC314
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This advanced course, recommended for anyone embarking on multicolor flow cytometry experiments, details the features of fluorescent probes typically used for flow cytometry. It teaches how to select the right combination of these fluorochromes for your studies and how to perform proper compensation. The course concludes with a demo of compensation adjustments on a DiVa-based instrument (LSRII or Fortessa) and on several analysis programs.

Next Class:
Thursday October 11, 2018
1pm-3:30pm
Grossberg Classroom CCC314
SIGN UP

 

Designed for newcomers to the software, this class will teach all basic operations for data analysis and presentation through hands-on instruction.

Next Class: 
September, 2018
12pm-1:30pm
Grossberg Classroom CCC314
SIGN UP