The Flow Sorting Facility houses two cell sorter instruments that are available for operator-assisted sorting, each with a slightly different configuration, but otherwise similar capabilities. The BD FACSAria I was upgraded in 2010 and is equipped with 12 fluorescent PMTs and 405, 488, 561, and 640 nm lasers, and has bio-containment capabilities. The FACSAria II is equipped with four lasers (355 nm, 405 nm, 488 nm, and 633 nm) and has 13 fluorescent detectors. Both instruments have the capacity to do four-way sorts as well as sorts into plates and onto slides. Our Aria instruments are in a BSL2+ facility.
The instruments are maintained and operated by dedicated facility staff members and are available during regular business hours by reservation. In a typical workflow, researchers will bring their stained single-cell suspensions to the core along with controls, buffers, and collection tubes. Then, together with the operator, the experiment is set up and sort logic is defined. After setup, researchers will not need to stay present for the remainder of the sort duration. There are training options for individuals who would like to run these sorters unassisted.
A third, more user-friendly flow cytometry sorter, the SONY MA900, is available for unassisted use 24/7 following a brief instructional session with one of our staff
The sorter schedules can be found on our FOM calendar, and an active FOM account is required for access to the sorters. Reservations for the FACSAria sorters can only be scheduled by the sorter operators, after submission of a Sort Request Form. Please email the completed Sort Request Form to Kitty. Investigators new to sorting or embarking on a new project are encouraged to discuss their goals with the staff before a first sort is performed.
Detailed information pertaining to our operation routine, reservations, cancellation policies, sample requirements, and collection options can be found in our document “Guidelines to Sorting”.
In addition to flow sorting capabilities, our facility also hosts a Miltenyi Classic AutoMACS for automated magnetic cell sorting. Reservations for this instrument are available to all trained researchers through FOM.