Bioanalytics, Metabolomics & Pharmacokinetics (BMPK) Services and Fees

Services

The Bioanalytics, Metabolomics & Pharmacokinetics (BMPK) Shared Resource is available to assist investigators in the design of their clinical and research studies incorporating clinical pharmacology, pharmacokinetic and pharmacodynamic objectives. Emphasis is placed on designing studies that optimize efficiency and scientific rigor. To achieve these objectives, BMPK offers assistance with the development and validation/qualification of new assay methodologies, sample analysis, and PK/PD data analysis and modeling. Investigators are encouraged to consult the BMPK Shared Resource as early as possible during the planning phases of their project or grant development.

Example Categories of Drug/Metabolite and Other Small Molecule Assays:

  • Hormones: Testosterone, dihydrotestosterone, androstenedione, dehydroepiandrosterone (DHEA), and androsterone (LC-MS/MS), and estrone and estradiol (LC-MS/MS)
  • Antimetabolites: Gemcitabine and dFdU (LC-MS/MS), capecitabine (LC-MS/MS), and 5-fluorouracil (LC-MS/MS)
  • Taxanes: Docetaxel (LC-MS/MS) and paclitaxel (LC-MS/MS)
  • Topoisomerase Agents: Irinotecan (CPT-11), SN-38, SN-38G (UPLC and LC-MS/MS)
  • Anthracyclines: Doxorubicin (UPLC) and daunorubicin (UPLC)
  • Targeted Agents : Tivozanib (LC-MS/MS), enzalutamide and N-desmethylenzalutamide (LC-MS/MS), sorafenib and sorafenib-N-oxide (LC-MS/MS), carfilzomib (LC-MS/MS), and sunitinib (LC-MS/MS)
  • Platinum Based Compounds: Oxaliplatin (atomic absorption), cisplatin (atomic absorption), and carboplatin (atomic absorption)
  • Selenium Based Compounds: Selenomethionine and methylselenocysteine (atomic absorption)
  • Others: Tryptophan/kynurenine (LC-MS/MS), sex hormone binding globulin (SHBG; CMIA), VEGFR2 (ELISA), lignans (enterodiol and enterolactone; LC-MS/MS) and eicosanoids (LC-MS/MS)
  • For a complete listing and additional specifications, refer to the table below

Fees

Collaborative interaction is essential to achieve optimal experimental design and outcomes. As a client of the Roswell Park BMPK shared resource, we'll arrange a one-on-one consultation with you to review your experimental design, method development needs, sample analysis strategy, and results interpretation in order to best meet your study aims and objectives. Over the course of the study, we'll work closely with you to ensure that you receive the most pertinent and reliable outcomes possible. As a result, pricing varies with experimental design, turnaround time, the number of analytes as well as the number of samples.

Investigators who submit samples to BMPK are required to fill out a BMPK Sample Submission Form Samples will not be analyzed without a sample submission form. Investigators interested in the use of the BMPK Shared Resource should contact Joshua Prey to discuss scheduling, shared resource procedures, and pricing.

Joshua Prey, MS
Research Project Administrator, BMPK Shared Resource
Center for Genetics and Pharmacology, L1-318
Phone: 716-845-3313
Email: joshua.prey@roswellpark.org

Analyte(s) Lower Limit of Quantitation
(LLOQ)
Calibration Range Methodology Status Matrices
Androgens (5)
Androstenedione
Testosterone
Dihydrotestosterone Dehydroepiandrosterone
Androsterone
6.25 pg/mL
6.25 pg/mL
12.5 pg/mL
200 pg/mL
200 pg/mL
0.00625 - 3.75 ng/mL
0.00625 - 3.75 ng/mL
0.0125 - 7.50 ng/mL
0.200 - 7.50 ng/mL
0.200 - 7.50 ng/mL
LC-MS/MS Validated Mouse Serum
Human Heparinized Plasma
Human Serum
Calcitriol 10.0 ng/mL 10.0 - 1,000 ng/mL LC-MS/MS Validated Drug Formulation
Carfilzomib 0.400 ng/mL 0.400 - 1,000 ng/mL LC-MS/MS Validated Human Heparinized Plasma
Ceritinib 1.00 ng/mL 1.00 - 500 ng/mL LC-MS/MS Validated Human Plasma
CFAK-C4 2.50 pg/mL 2.50 - 2,500 pg/mL LC-MS/MS Qualified Dog Heparinized Plasma
CPT-11 (Irinotecan) 2.50 ng/mL 2.50 - 1,000 ng/mL UPLC / Fluorescence Qualified Human Plasma
Cytarabine 1.00 ng/mL 1.00 - 1,000 ng/mL LC-MS/MS Qualified Human Plasma
Docetaxel 0.200 ng/mL 0.200 - 400 ng/mL LC-MS/MS Validated Human Plasma
Docetaxel
Docetaxel M1
Docetaxel M2
Docetaxel M3
Docetaxel M4
5.00 ng/mL
5.00 ng/mL
5.00 ng/mL
5.00 ng/mL
7.50 ng/mL
5.00 - 1,000 ng/mL
5.00 - 1,000 ng/mL
5.00 - 1,000 ng/mL
5.00 - 1,000 ng/mL
7.50 - 1,500 ng/mL
LC-MS/MS Qualified Mouse Plasma
Rat Plasma
Dog Plasma
Human Plasma
Dovitinib 2.00 ng/mL 2.00 - 1,000 ng/mL LC-MS/MS Qualified Human Heparinized Plasma
Eicosanoids (7)
Prostaglandin D2
Prostaglandin E2
13-Hydroxyoctadecadienoic acid
5-Hydroxyeicosatetraenoic acid
12-Hydroxyeicosatetraenoic acid
15-Hydroxyeicosatetraenoic acid
Arachidonic Acid
1.00 ng/mL 1.00 - 400 ng/mL LC-MS/MS Qualified Mouse Xenografts
Enterodiol
Enterolactone
0.500 ng/mL
0.500 ng/mL
0.500 - 150 ng/mL
0.500 - 150 ng/mL
LC-MS/MS Validated Human Serum
Entinostat 0.500 ng/mL 0.500 - 250 ng/mL LC-MS/MS Qualified Human Plasma
Enzalutamide
N-Desmethyl Enzalutamide
10.0 ng/mL
10.0 ng/mL
10.0 - 20,000 ng/mL
10.0 - 20,000 ng/mL
LC-MS/MS Validated Human EDTA Plasma
Enzalutamide
N-Desmethyl Enzalutamide
10.0 ng/mL
10.0 ng/mL
10.0 - 20,000 ng/mL
10.0 - 20,000 ng/mL
LC-MS/MS Qualified Woodchuck EDTA Plasma
Epacadostat 5.00 ng/mL 5.00 - 1,000 ng/mL LC-MS/MS Validated Human EDTA Plasma
Estrone and
17ß-Estradiol
2.00 pg/mL
1.00 pg/mL
2.00 - 250 pg/mL
1.00 - 250 pg/mL
LC-MS/MS Validated Human Heparinized Plasma
Human Serum
FL118 1.00 ng/mL 1.00 - 500 ng/mL UPLC / Fluorescence Qualified Human Plasma
Gemcitabine
dFdU
1.00 ng/mL
5.00 ng/mL
1.00 - 2,000 ng/mL
5.00 - 10,000 ng/mL
LC-MS/MS Validated Human Plasma
Gemcitabine
dFdU
Capecitabine
5-Fluorouracil
5-DFCR
5-DFUR
1.00 ng/mL
1.00 ng/mL
1.00 ng/mL
5.00 ng/mL
5.00 ng/mL
5.00 ng/mL
1.00 - 1,000 ng/mL
1.00 - 1,000 ng/mL
1.00 - 1,000 ng/mL
5.00 - 1,000 ng/mL
5.00 - 1,000 ng/mL
5.00 - 1,000 ng/mL
LC-MS/MS Qualified Human Plasma
Irinotecan (CPT-11) 2.50 ng/mL 2.50 - 1,000 ng/mL UPLC / Fluorescence Qualified Human Plasma
JP-11646 1.00 ng/mL 1.00 - 500 ng/mL LC-MS/MS Validated Mouse Plasma
Rat Plasma
Dog Plasma
Mannitol
Lactulose
3-MGP
0.980 µg/mL
0.980 µg/mL
2.93 µg/mL
0.980 - 62.5 µg/mL
0.980 - 62.5 µg/mL
2.93 - 62.5 µg/mL
LC-MS/MS Qualified Human Plasma
Molybdenum 10.0 ng/mL 10.0 - 1,000 ng/mL Atomic Absorption Qualified Human Plasma
Nicotine
Cotinine
Hydroxy Cotinine
Cotinine Glucuronide
Anatabine
Atabasine
1.00 ng/mL
1.00 ng/mL
1.00 ng/mL
10.0 ng/mL
1.00 ng/mL
1.00 ng/mL
1.00 - 100 ng/mL
1.00 - 100 ng/mL
1.00 - 100 ng/mL
10.0 - 500 ng/mL
1.00 - 100 ng/mL
1.00 - 100 ng/mL
LC-MS/MS Qualified Human Urine
Nintedanib 0.500 ng/mL 0.500 - 250 ng/mL LC-MS/MS Validated Human EDTA Plasma
Paclitaxel and
6α-Hydroxy Paclitaxel
2.00 ng/mL
2.00 ng/mL
2.00 - 1,000 ng/mL
2.00 - 1,000 ng/mL
LC-MS/MS Validated Human Plasma
Pemetrexed 5.00 ng/mL 5.00 - 2,000 ng/mL LC-MS/MS Qualified Human Plasma
Platinum 250 ng/mL 250 - 8,000 ng/mL Atomic Absorption Qualified Drug Formulation
Human Plasma
Rapamycin 1.25 ng/mL 1.25 - 100 ng/mL LC-MS/MS Validated Human Plasma
Selenium 70.0 ng/mL 70.0 - 1,200 ng/mL Atomic Absorption Validated Human Plasma
Sex Hormone Binding Globulin (SHBG) 2.00 nmol/L 2.00 - 250 nmol/L Chemiluminescent microparticle immunoassay Validated Human Serum
SN-38
SN-38G
0.200 ng/mL
0.200 ng/mL
0.200 - 1,000 ng/mL UPLC / Fluorescence Qualified Human Plasma
Sorafenib
Sorafenib N-oxide
50.0 ng/mL
12.5 ng/mL
50.0 - 10,000 ng/mL
12.5 - 2,500 ng/mL
LC-MS/MS Validated Human Plasma
Sorafenib
Sorafenib N-oxide
50.0 ng/mL
12.5 ng/mL
50.0 - 10,000 ng/mL
12.5 - 2,500 ng/mL
LC-MS/MS Qualified Woodchuck Plasma
(Z)-Tamoxifen
(Z)-N-Desmethyltamoxifen
(Z)-4-Endoxifen
(Z)-4-Hydoxytamoxifen
1.00 ng/mL
1.00 ng/mL
0.500 ng/mL
0.250 ng/mL
1.00 - 400 ng/mL
1.00 - 400 ng/mL
0.500 - 200 ng/mL
0.250 - 100 ng/mL
LC-MS/MS Validated Human Plasma
Human Breast Tissue
Tivozanib 0.500 ng/mL 0.500 - 150 ng/mL LC-MS/MS Validated Human EDTA Plasma
Tryptophan and Kynurenine 40.0 ng/mL
10.0 ng/mL
40.0 - 20,000 ng/mL
10.0 - 5,000 ng/mL
LC-MS/MS Validated Human Plasma
VEGFR2 80.0 pg/mL 80.0 - 5,000 pg/mL ELISA Validated Human EDTA Plasma