Bioanalytics, Metabolomics & Pharmacokinetics (BMPK) Services and Fees

Services

The Bioanalytics, Metabolomics & Pharmacokinetics (BMPK) Shared Resource is available to assist investigators in the design of their clinical and research studies incorporating clinical pharmacology, pharmacokinetic and pharmacodynamic objectives. Emphasis is placed on designing studies that optimize efficiency and scientific rigor. To achieve these objectives, BMPK offers assistance with the development and validation/qualification of new assay methodologies, sample analysis, and PK/PD data analysis and modeling. Investigators are encouraged to consult the BMPK Shared Resource as early as possible during the planning phases of their project or grant development.

Example Categories of Drug/Metabolite and Other Small Molecule Assays:

  • Hormones: Testosterone, dihydrotestosterone, androstenedione, dehydroepiandrosterone (DHEA), and androsterone (LC-MS/MS), and estrone and estradiol (LC-MS/MS)
  • Antimetabolites: Gemcitabine and dFdU (LC-MS/MS), capecitabine (LC-MS/MS), and 5-fluorouracil (LC-MS/MS)
  • Taxanes: Docetaxel (LC-MS/MS) and paclitaxel (LC-MS/MS)
  • Topoisomerase Agents: Irinotecan (CPT-11), SN-38, SN-38G (UPLC and LC-MS/MS)
  • Anthracyclines: Doxorubicin (UPLC) and daunorubicin (UPLC)
  • Targeted Agents : Tivozanib (LC-MS/MS), enzalutamide and N-desmethylenzalutamide (LC-MS/MS), sorafenib and sorafenib-N-oxide (LC-MS/MS), carfilzomib (LC-MS/MS), and sunitinib (LC-MS/MS)
  • Platinum Based Compounds:  Oxaliplatin (atomic absorption), cisplatin (atomic absorption), and carboplatin (atomic absorption)
  • Selenium Based Compounds:  Selenomethionine and methylselenocysteine (atomic absorption)
  • Others: Tryptophan/kynurenine (LC-MS/MS), sex hormone binding globulin (SHBG; CMIA), VEGFR2 (ELISA), lignans (enterodiol and enterolactone; LC-MS/MS) and eicosanoids (LC-MS/MS)
  • For a complete listing and additional specifications, refer to the table below

Fees

Collaborative interaction is essential to achieve optimal experimental design and outcomes. As a client of the Roswell Park BMPK shared resource, we'll arrange a one-on-one consultation with you to review your experimental design, method development needs, sample analysis strategy, and results interpretation in order to best meet your study aims and objectives.  Over the course of the study, we'll work closely with you to ensure that you receive the most pertinent and reliable outcomes possible. As a result, pricing varies with experimental design, turnaround time, the number of analytes as well as the number of samples. 

Investigators who submit samples to BMPK are required to fill out a . Samples will not be analyzed without a sample submission form. Investigators interested in the use of the BMPK Shared Resource should contact Joshua Prey to discuss scheduling, shared resource procedures, and pricing.

 

Joshua Prey, MS
Research Project Administrator, BMPK Shared Resource
Center for Genetics and Pharmacology, L1-318
Phone: 716-845-3313
Email: joshua.prey@roswellpark.org

 

Analyte(s)

Lower Limit of Quantitation
(LLOQ)

Calibration Range

Methodology

Status

Matrices

Androgens (5)
Androstenedione
Testosterone
Dihydrotestosterone Dehydroepiandrosterone
Androsterone

6.25 pg/mL
6.25 pg/mL
12.5 pg/mL
200 pg/mL
200 pg/mL

0.00625 - 3.75 ng/mL
0.00625 - 3.75 ng/mL
0.0125 - 7.50 ng/mL
0.200 - 7.50 ng/mL
0.200 - 7.50 ng/mL

LC-MS/MS

Validated

Mouse Serum
Human Heparinized Plasma
Human Serum

Calcitriol

10.0 ng/mL

10.0 - 1,000 ng/mL

LC-MS/MS

Validated

Drug Formulation

Carfilzomib

0.400 ng/mL

0.400 - 1,000 ng/mL

LC-MS/MS

Validated

Human Heparinized Plasma

Ceritinib

1.00 ng/mL

1.00 - 500 ng/mL

LC-MS/MS

Validated

Human Plasma

CFAK-C4

2.50 pg/mL

2.50 - 2,500 pg/mL

LC-MS/MS

Qualified

Dog Heparinized Plasma

CPT-11 (Irinotecan)

2.50 ng/mL

2.50 - 1,000 ng/mL

UPLC/Fluorescence

Qualified

Human Plasma

Cytarabine

1.00 ng/mL

1.00 - 1,000 ng/mL

LC-MS/MS

Qualified

Human Plasma

Docetaxel

0.200 ng/mL

0.200 - 400 ng/mL

LC-MS/MS

Validated

Human Plasma

Docetaxel
Docetaxel M1
Docetaxel M2
Docetaxel M3
Docetaxel M4

5.00 ng/mL
5.00 ng/mL
5.00 ng/mL
5.00 ng/mL
7.50 ng/mL

5.00 - 1,000 ng/mL
5.00 - 1,000 ng/mL
5.00 - 1,000 ng/mL
5.00 - 1,000 ng/mL
7.50 - 1,500 ng/mL

LC-MS/MS

Qualified

Mouse Plasma
Rat Plasma
Dog Plasma
Human Plasma

Dovitinib

2.00 ng/mL

2.00 - 1,000 ng/mL

LC-MS/MS

Qualified

Human Heparinized Plasma

Eicosanoids (7)
Prostaglandin D2
Prostaglandin E2
13-Hydroxyoctadecadienoic acid
5-Hydroxyeicosatetraenoic acid
12-Hydroxyeicosatetraenoic acid
15-Hydroxyeicosatetraenoic acid
Arachidonic Acid

1.00 ng/mL

1.00 - 400 ng/mL

LC-MS/MS

Qualified

Mouse Xenografts

Enterodiol
Enterolactone

0.500 ng/mL
0.500 ng/mL

0.500 - 150 ng/mL
0.500 - 150 ng/mL

LC-MS/MS

Validated

Human Serum

Entinostat

0.500 ng/mL

0.500 - 250 ng/mL

LC-MS/MS

Qualified

Human Plasma

Enzalutamide
N-Desmethyl Enzalutamide

10.0 ng/mL
10.0 ng/mL

10.0 - 20,000 ng/mL
10.0 - 20,000 ng/mL

LC-MS/MS

Validated

Human EDTA Plasma

Enzalutamide
N-Desmethyl Enzalutamide

10.0 ng/mL
10.0 ng/mL

10.0 - 20,000 ng/mL
10.0 - 20,000 ng/mL

LC-MS/MS

Qualified

Woodchuck EDTA Plasma

Epacadostat

5.00 ng/mL

5.00 - 1,000 ng/mL

LC-MS/MS

LC-MS/MS

Validated

Human EDTA Plasma

Estrone and
17ß-Estradiol

2.00 pg/mL
1.00 pg/mL

2.00 - 250 pg/mL
1.00 - 250 pg/mL

LC-MS/MS

Validated

Human Heparinized Plasma
Human Serum

FL118

1.00 ng/mL

1.00 - 500 ng/mL

UPLC/Fluorescence

Qualified

Human Plasma

Gemcitabine
dFdU

1.00 ng/mL
5.00 ng/mL

1.00 - 2,000 ng/mL
5.00 - 10,000 ng/mL

LC-MS/MS

Validated

Human Plasma

Gemcitabine
dFdU
Capecitabine
5-Fluorouracil
5-DFCR
5-DFUR

1.00 ng/mL
1.00 ng/mL
1.00 ng/mL
5.00 ng/mL
5.00 ng/mL
5.00 ng/mL

1.00 - 1,000 ng/mL
1.00 - 1,000 ng/mL
1.00 - 1,000 ng/mL
5.00 - 1,000 ng/mL
5.00 - 1,000 ng/mL
5.00 - 1,000 ng/mL

LC-MS/MS

Qualified

Human Plasma

Irinotecan (CPT-11)

2.50 ng/mL

2.50 - 1,000 ng/mL

UPLC/Fluorescence

Qualified

Human Plasma

JP-11646

1.00 ng/mL

1.00 - 500 ng/mL

LC-MS/MS

Validated

Mouse Plasma
Rat Plasma
Dog Plasma

Mannitol
Lactulose
3-MGP

0.980 µg/mL
0.980 µg/mL
2.93 µg/mL

0.980 - 62.5 µg/mL
0.980 - 62.5 µg/mL
2.93 - 62.5 µg/mL

LC-MS/MS

Qualified

Human Plasma

Molybdenum

10.0 ng/mL

10.0 - 1,000 ng/mL

Atomic Absorption

Qualified

Human Plasma

Nicotine
Cotinine
Hydroxy Cotinine
Cotinine Glucuronide
Anatabine
Atabasine

1.00 ng/mL
1.00 ng/mL
1.00 ng/mL
10.0 ng/mL
1.00 ng/mL
1.00 ng/mL

1.00 - 100 ng/mL
1.00 - 100 ng/mL
1.00 - 100 ng/mL
10.0 - 500 ng/mL
1.00 - 100 ng/mL
1.00 - 100 ng/mL

LC-MS/MS

Qualified

Human Urine

Nintedanib

0.500 ng/mL

0.500 - 250 ng/mL

LC-MS/MS

Validated

Human EDTA Plasma

Paclitaxel and
6α-Hydroxy Paclitaxel

2.00 ng/mL
2.00 ng/mL

2.00 - 1,000 ng/mL
2.00 - 1,000 ng/mL

LC-MS/MS

Validated

Human Plasma

Pemetrexed

5.00 ng/mL

5.00 - 2,000 ng/mL

LC-MS/MS

Qualified

Human Plasma

Platinum

250 ng/mL

250 - 8,000 ng/mL

Atomic Absorption

Qualified

Drug Formulation
Human Plasma

Rapamycin

1.25 ng/mL

1.25 - 100 ng/mL

LC-MS/MS

Validated

Human Plasma

Selenium

70.0 ng/mL

70.0 - 1,200 ng/mL

Atomic Absorption

Validated

Human Plasma

Sex Hormone Binding Globulin (SHBG)

2.00 nmol/L

2.00 - 250 nmol/L

Chemiluminescent microparticle immunoassay

Validated

Human Serum

SN-38
SN-38G

0.200 ng/mL
0.200 ng/mL

0.200 - 1,000 ng/mL

UPLC/Fluorescence

Qualified

Human Plasma

Sorafenib
Sorafenib N-oxide

50.0 ng/mL
12.5 ng/mL

50.0 - 10,000 ng/mL
12.5 - 2,500 ng/mL

LC-MS/MS

Validated

Human Plasma

Sorafenib
Sorafenib N-oxide

50.0 ng/mL
12.5 ng/mL

50.0 - 10,000 ng/mL
12.5 - 2,500 ng/mL

LC-MS/MS

Qualified

Woodchuck Plasma

(Z)-Tamoxifen
(Z)-N-Desmethyltamoxifen
(Z)-4-Endoxifen
(Z)-4-Hydoxytamoxifen

1.00 ng/mL
1.00 ng/mL
0.500 ng/mL
0.250 ng/mL

1.00 - 400 ng/mL
1.00 - 400 ng/mL
0.500 - 200 ng/mL
0.250 - 100 ng/mL

LC-MS/MS

Validated

Human Plasma
Human Breast Tissue

Tivozanib

0.500 ng/mL

0.500 - 150 ng/mL

LC-MS/MS

Validated

Human EDTA Plasma

Tryptophan and Kynurenine

40.0 ng/mL
10.0 ng/mL

40.0 - 20,000 ng/mL
10.0 - 5,000 ng/mL

LC-MS/MS

Validated

Human Plasma

VEGFR2

80.0 pg/mL

80.0 - 5,000 pg/mL

ELISA

Validated

Human EDTA Plasma