Bioanalytics, Metabolomics & Pharmacokinetic Facility Data for Grants

The BMPK Shared Resource provides bioanalytical, modeling and consultation services to support discovery-based research and pre-clinical/clinical research and drug development. These services range from study design and methodology consultation, for formulation development and preparation, sample storage and retrieval preparation and assay of study samples, data compilation and analysis, and PK/PD modeling. Collaborations between BMPK and CCSG investigators, faculty, and staff are conducted through one-on-one meetings to prospectively plan and develop projects or grants, or to discuss and evaluate study conduct, milestones and /or outcomes on an as-needed basis. The resource offers a wide variety of analytical methods as well as capabilities to develop and validate new assays. BMPK provides highly sensitive measurements for numerous chemotherapeutic agents and their metabolites as well as endogenous compounds in a wide variety of sample matrices (e.g., whole blood, plasma, serum, urine, cell pellets and media, xenografts, and large variety of tissue types from biodistribution studies) using state- of-the-art techniques of LC-MS, LC- MS/MS, UPLC, HPLC, ELISA and Atomic Absorption spectrophotometry.

Non-compartmental analysis (NCA) and PK/PD modeling and simulations are performed to gain insight into the life cycle and mechanism of action of cancer therapeutics as singular agents or in combination with other compounds, and to assess inter-individual and random variability within study populations. The information gained from past studies is used to optimize the design aspects of future clinical trials including dosing strategy and the selection of patient populations. The BMPK Shared Resource serves as an essential component of CCSG’s research effort developing complementary collaborations between its bioanalytical and pharmacokinetic skillsets and other shared resources such as genomics, biostatistics, laboratory animal, hematology, etc.

BMPK Instrumentation

  • Applied Biosystems 5500 QTrap Triple quadrupole ESI/APCI-LC/MS/MS
  • Applied Biosystems 5500 Triple quadrupole ESI/APCI-LC/MS/MS
  • Thermo Scientific TSQ Vantage triple quadrupole ESI/APCI-LC/MS/MS
  • Applied Biosystems API3000 triple quadrupole DES LC/MS/MS
  • Waters Ultra Performance Liquid Chromatography (UPLC) System
  • Waters Alliance High Performance Liquid Chromatography (HPLC) System
  • Perkin Elmer Analyst 600 Graphite Furnace Atomic Absorption
  • Biotek Synergy Plate Reader providing fluorescence and UV detection
  • TomTec Quadra 4 Robotic Handling System

BMPK Modeling Software

  • Phoenix WinNonlin Professional: Non-compartmental and compartmental modeling
  • ADAPT 5 and S ADAPT: individual compartmental PK and PK/PD modeling
  • NONMEN: nonlinear mixed effect modeling software for population analysis to determine sources of variability in the PK and PD of a drug
  • WinPOPT: provides information for PK and /or PK/PS sampling strategies
  • SAS 9.3: used to manage and clean databases, and create graphics and tables
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