We are currently studying the role of mitochondria in cancer. Mitochondria dysfunction is associated with tumorigenesis and resistance to cancer therapy. The mechanisms underlying these outcomes remain undefined. We have recently developed a novel method that defines mitochondria structure and function at single cell level.
These findings have significance in early detection of cancerous cells and monitoring the physiological status of single cell during cancer therapy. We study pharmacological agents to modify mitochondria function and improve cancer cell death. We utilize various biochemical and molecular approaches to interrogate cell culture and mouse models of human cancer.
Yadav N, Pliss A, Kuzmin A, Rapali P, Sun L, Prasad P, Chandra D. Transformations of the macromolecular landscape at mitochondria during DNA-damage-induced apoptotic cell death. Cell Death Dis. 2014 Oct 9;5:e1453.
Yadav N, Chandra D. Mitochondrial DNA mutations and breast tumorigenesis. Biochim Biophys Acta. 2013 Dec;1836(2):336-44.
Gogada R*, Yadav N*, Liu J, Tang S, Zhang D, Schneider A, Seshadri A, Sun L, Aldaz CM, Tang DG, Chandra D. Bim, a proapoptotic protein, up-regulated via transcription factor E2F1-dependent mechanism, functions as a prosurvival molecule in cancer. J Biol Chem. 2013 Jan 4;288(1):368-81 (* Equal contribution).
Jackson CA, Yadav N, Min S, Li J, Milliman EJ, Qu J, Chen YC, Yu MC. Proteomic analysis of interactors for yeast protein arginine methyltransferase Hmt1 reveals novel substrate and insights into additional biological roles. Proteomics. 2012 Nov;12(22):3304-14.
Yadav N, Cheng D, Richard S, Morel M, Iyer VR, Aldaz CM, Bedford MT. CARM1 promotes adipocyte differentiation by coactivating PPARgamma. EMBO Rep. 2008 Feb;9(2):193-8.
Cheng D, Yadav N, King RW, Swanson MS, Weinstein EJ, Bedford MT. Small molecule regulators of protein arginine methyltransferases. J Biol Chem. 2004 Jun 4;279(23):23892-9.
Yadav N, Lee J, Kim J, Shen J, Hu MC, Aldaz CM, Bedford MT. Specific protein methylation defects and gene expression perturbations in coactivator-associated arginine methyltransferase 1-deficient mice. Proc Natl Acad Sci U S A. 2003 May 27;100(11):6464-8.