RPCI - 43 Male Chimpanzee BAC Library
The RPCI-43 BAC Library has been constructed in our laboratory by Chung-Li Shu. DNA was isolated from white blood cells obtained from a male chimpanzee (Pan troglodytes, "Clinton", Yerkes #C0471; birthdate: 6-6-80). The agarose-embedded DNA was partially digested with a combination of EcoRI and EcoRI Methylase. Size selected DNA was cloned into the pBACe3.6 vector between the EcoRI sites. The ligation products were transformed into DH10B electrocompetent cells (BRL Life Technologies). The library has been arrayed into 384-well microtiter dishes and also gridded onto 22x22cm nylon high-density filters for screening by probe hybridization. Each hybridization membrane represents over 18,000 distinct chimpanzee BAC clones, stamped in duplicate.
The Total RPCI-43 Male Chimpanzee BAC Library:
| Segment | Cloning Vector | DNA | Plate Numbers | Total Plates | Total Clones | Total Empty Wells |
| 1 | pBACe3.6 | Male WBC | 1-185 | 185 | 68721 | 2319 |
| Segment | (%) Empty Wells | Total Non-Recombinant Clones | % Non-Recombinant Clones | Average Insert Size (Kb) | Genomic Coverage |
| 1 | 3.3 | approx. 960 | 1.3 | 157 | 3.5X |
click here for a legend of the previous tables.
The total library should represent approximately 3.5-fold total genomic representation.
The RPCI-43 Male Chimpanzee BAC Library is available both as library plates and high-density hybridization filter sets.
Data on the RPCI-43 clone average insert size has been determined by Pulsed Field Gel Electriphoresis. Clone size distribution has been plotted graphically. Further (in depth) characterization of the library is ongoing in our lab and data will be updated on our web page periodically.