The RPCI-81 Canine BAC Library has been constructed at RPCI by Robin Li (Stanford University). The library constructed was collaborative effort that included support from the following groups: Emmanual Mignot (Stanford University), Donald Patterson (University of Pennsylvania), Gus Agiurre (Cornell University), Elaine Ostrander (University of Washington, Seattle), and Bernard van Oost (University of Utrecht, the Netherlands).
Male Doberman Pinscher dog blood genomic DNA, partially digested with EcoRI/EcoRI Methylase, was cloned into the pBACe3.6 vector. The ligation products were transformed into DH10B electrocompetent cells (BRL Life Technologies). The library has been arrayed into 384-well microtiter dishes and gridded onto 22x22cm with EcoRI/EcoRI Methylase, was cloned into the pBACe3.6 nylon high density hybridization filters for screening purposes.
The Total RPCI-81 Canine BAC Library:
| Cloning Vector | DNA | Plate Numbers | Total Plates | Total Clones Picked | Total Empty Wells | % Empty Wells |
| pBACe3.6 | Doberman Pinscher (Blood) | 1-432 | 432 | 165888 | 5473 | 3.3 |
| Total Non-Recombinant Clones | % Non-Recombinant Clones | Total Clones | Average Insert Size (Kb) | Genomic Coverage |
| 3318 | 2.0 | 157255 | 155 | 8.1 |
click here for a legend of the previous tables.
The average insert size data and clone size distribution has been determined by Pulsed Field Gel Electriphoresis analyses of random clones chosen from library plates spanning the entire RPCI-81 library. Further (in depth) characterization is ongoing and data will be updated on this WebPage periodically.