The RPCI-41 BAC Library has been constructed at RPCI by Baohui Zhao. White blood cell genomic DNA from a male Olive Baboon was isolated and partially digested with a combination of EcoRI and EcoRI Methylase. Size selected DNA was cloned into the pBACe3.6 vector between the EcoRI sites. The ligation products were transformed into DH10B electro-competent cells (BRL LIFE TECHNOLOGIES). The library has been arrayed into 384-well microtiter dishes and also gridded onto 22x22cm nylon high-density filters for screening by probe hybridization. Each hybridization membrane represents over 18,000 distinct baboon BAC clones, stamped in duplicate.
The animal ("1X3655") used as the sample donor for this BAC library was born in the Southwest Foundation for Biomedical Research baboon colony in April 1980. For additional information, contact Dr. Jeffery Rogers by Email, or FAX at:
Dept. of Genetics
Southwest Foundation for Biomedical Research
P.O. Box 760549
San Antonio, Texas 78245
FAX: 210-670-3316
Email: jrogers@darwin.sfbr.org
The RPCI - 41 Male Olive Baboon BAC Library:
Segment |
DNA |
Plate Numbers |
Total Plates |
Total Clones |
Empty Wells (Total) |
|
1 |
pBACe3.6 |
Male WBC |
1-240 |
240 |
89,536 |
2,074 |
2 |
pBACe3.6 |
Male WBC |
241-480 |
240 |
90,468 |
1,602 |
Total Library |
480 |
180,004 |
3,676 |
Segment |
Empty Wells (%) |
Non-Recombinant Clones(Total) |
Non-Recombinant Clones(%) |
Average Insert Size |
Genomic Coverage |
1 |
2.3 |
approx. 550 |
0.6 |
177 Kbp |
5.2X |
2 |
1.7 |
approx. 90 |
0.1 |
173 Kbp |
5.2X |
Total Library |
2.0 |
approx. 640 |
0.3 |
175 Kbp |
10.4X |
click here for a legend of the previous tables.
This library should represent approximately 10.4-fold total genomic representation.
For laboratories not requiring all 10.4-fold genomic representation, each segment of the RPCI-41 Male Baboon BAC Library is available on an individual basis both as library plates and high-density hybridization sets.
Data on the RPCI-41 clone average insert size has been determined by Pulsed Field Gel Electrophoresis. Clone size distribution has been plotted graphically. Further (in depth) characterization of the library is on going in our lab and data will be updated on our web page periodically.