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SKY Resource
SKY/FISH Analysis Resource
Sei-Ichi Matsui, Ph.D.
Director, SKY/FISH Facility
Roswell Park Cancer Institute
Elm and Carlton Streets
Buffalo, New York 14263
Telephone:(716)845-3213
Fax: (716)845-1698
Email: Seiichi.Matsui@RoswellPark.org
Location
Office and Laboratory: Buffalo Life Science Complex
Office: BLSC L2-302
Laboratory: BLSC L2-106
Microscope Rooms: BLSC L2-204 and 205
Culture Facility: BLSC L2-202
Scheduling
Weekdays- 8:30 a.m. – 5:30 p.m.
Sei-Ichi Matsui, Ph.D.
Director
Office - BLSC L2-302
Telephone: (716)845-3213
Fax: (716)845-1698
E-mail: Seiichi.Matsui@RoswellPark.org
Jeffrey LaDuca, M.Sc.
Technologist
Laboratory Location - BLSC L2-106
Telephone: (716)845-8543
Fax: (716)845-1698
E-mail: Jeffrey.Laduca@RoswellPark.org
Equipment/Infrastructure
- Two Nikon Fluorescence Microscopes equipped with six sets of Fluorescence Signal-Decipher Filters
- Nikon T2000 Eclipse inverted microscope with phase contrast and bright-field lenses and image-capturing camera head
- Spectral Karyotyping (SKY) SD300VDS workstation with new SKYView® software with G-band Karyotyping capability for both human and mouse chromosomes. Acquisition software including SKYView Expo® and DVD/CD burner
- Spectral Karyotyping (SKY) SD-300 HAM Workstation
- SKYView analysis network
- Hybrite Apparatus (three units) for SKY and Fluorescent In Situ Hybridization (FISH)
- Image Storage/Retrieval Software (CD/DVD)
- EasyFISH Software analysis
- FISHView Workstation/Software analysis with Nikon Microscope
Services
1. Spectral Karyotype evaluation of leukemia, sold tumors and cell lines.
2. Evaluation of the dynamic role of chromosomes and genes in cancer cells.
3. Genomic validation of cells used in immunology, pharmacology and genetics.
4. Assessment of genomic and chromosomal instability.
5. Validation of transgenes, gene-knockout and gentically engineered cells
6. Quantificaiton of gene copy number in clinical and transgene research
7. Consultation, data interpretation, training and ducation.
User Fees
The user fee has been determined according to the Institute's Cost Sharing Policy. If the cost is an issue, please contact Dr. Sei-Ichi Matsui. The Administration will provide assistance.
Billing Procedures
Investigators are billed from the Administration - Central Billing Office for specific services rendered according to the rate schedule and are required to transfer the necessary funds to the SKY/FISH acccount (HRI 59-5100) established by Health Research, Inc. (Internal Users). For further billing details, please contact Ms. Wendy Molnar (716) 845-1534, Billing Administrator.
Rate Schedule - Guideline
All services include a full report, SKY or FISH images, interpretations and files in CD/DVD format, if desired.
SKY analysis (SKY-1 Human) | $150.00 | This rate is only available for joint projects with the SKY/FISH Facility and also for CCSG Members. |
| SKY analysis (SKY-1 Mouse) | $300.00 | This rate is only available for joint projects with the SKY/FISH Facility and also for CCSG members. |
| SKY analysis (SKY-2 Human) | $500.00 | Other than SKY-1 projects. |
| SKY analysis (SKY-2 Mouse) | $750.00 | Other than SKY-1 projects. |
| FISH analysis (FISH-1 Human) | $150.00 | One color FISH project. This rate is only available for joint projects with the SKY/FISH Facility and also for CCSG members. |
| FISH analysis (FISH-1-Mouse) | $150.00 | One color FISH project. This rate is only available for joint projects with the SKY/FISH Facility and also for CCSG members. |
| FISH analysis-(FISH-2 Human) | $300.00 | Two color FISH Projects. This rate is only available for joint projects with the SKY/FISH Facilty and also for CCSG members. |
| FISH analysis (FISH-2Mouse) | $300.00 | Two color FISH Projects. This rate is only available for joint projects with the SKY/FISH Facilty and also for CCSG members. |
| FISH analysis (FISH-3 Human) | $500.00 | One color FISH project. Other than FISH-1 and FISH-2 projects. |
| FISH analysis (FISH-3 Mouse) | $500.00 | One color FISH project. Other than FISH-1 and FISH-2 projects. |
| FISH analysis (FISH-4 Human) | $750.00 | Two color FISH projects. Other than FISH-1, FISH-2 and FISH-3 projects. |
| FISH analysis (FISH-4 Mouse) | $750.00 | Two color FISH projects. Other than FISH-1, FISH-2 and FISH-3 projects. |
SKY/FISH Analysis SKY/FISH-1 Human |
$500.00 |
Sequential SKY/FISH analysis. One color FISH project. This rate is only available for joint projects with the SKY/FISH facility and also for CCSG members. |
| SKY/FISH-1 Mouse | $750.00 | Sequential SKY/FISH analysis. One color FISH project. This rate is only available for joint projects with the SKY/FISH facility and also for CCSG members. |
| SKY/FISH-2 Human | $750.00 | Sequential SKY/FISH analysis. One color FISH project other than SKY/FISH-1 project. |
| SKY/FISH-2 Mouse | $1,000.00 | Sequential SKY/FISH analysis. One color FISH project other than SKY/FISH-1 project. |
| Culture and Cytogenetic Preparations | $200.00 | All projects |
| Consultation | No charge | All projects |
| Supplies (colcemide, chs, etc. | No charge | All projects |
FISH Analysis
Probes of interest (genes cloned in cosmid, plasmic, YAC, BAC, PAC, etc) have to be prepared by the customer. BAC and PAC clones are available from the DNA Microarray Resource (Dr. Norma Nowak 716-845-3361). Detection of ~ 1 kb sequences is possible. We will label the probe (free of charge) and run FISH. For three-color FISH, please contact Dr. Sei-Ichi Matsui 716-845-3213.
