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Gene Targeting and Transgenics Resource
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Contact info: |
Aimee Stablewski, MA Kenneth W. Gross, PhD, Co-Director |
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Description
The Gene Targeting and Transgenic Facility enables investigators to modify the murine genome by transgenic approaches for the systematic dissection of the genetic, molecular, cellular and physiological mechanisms underlying complex biological processes. Significant advances have been made in oncology, immunology, and developmental biology using this technology. We are highly sucessful in acheiving our goals to generate these animals, as demonstrated by our
RECORDS OF SUCCESS:
Location
Medical Research Complex, RPCI
Equipment
- Stoelting and de Fonbrune-type Microforge
- Sutter Pipette Puller and Pipette Beveler
- Narishighe Pipet Beveler
- Dissecting Microscopes (2)
- CO2 Incubator (3)
- Three Diaphot Inverted Microscopes with Micromanipulators
- Beckman L90-K ultracentrifuge
- SMZ-1 Nikon Stereomicroscopes (2)
Operating Schedule
Weekdays, 8 a.m. to 5:30 p.m.
Using the Facility
Investigators should contact Aimee Stablewski (aimee.stablewski@roswellpark.org)
Services
1) Production of transgenic mice via pronuclear injection
2) Production of chimeric mice via injection of modified ES cells into blastocysts
4) Rederivation
5) DNA purification
