NAME	SEQUENCE	BASE #	MW	TM
T7/T3/SP6/BGH/CMV T3 T3cs T7 SP6 BGH reverse CMV forward	ATT AAC CCT CAC TAA AG A ATT AAC CCT CAC TAA AGG G AAT ACG ACT CAC TAT AG ATT TAG GTG ACA CTA TAG AA TAG AAG GCA CAG TCG AGG CGC AAA TGG GCG GTA GGC GTG	17 20 17 20 18 21	5122 6092 5161 6161 5592 6553	40 48 40 44 50 60
Bluescript primers KS SK BL-REV	CTC GAG GTC GAC GGT ATC GA CGC TCT AGA ACT AGT GGA TC GGA AAC AGC TAT GAC CAT G	20 20 19	6152 6113 5842	56 52 49
pUC/M13 primers M13-48R M13-21 M13-20 M13-47 M13-reverse	AGC GGA TAA CAA TTT CAC ACA GGA T GTA AAA CGA CGG CCA GT GTA AAA CGA CGG CCA GT CGC CAG GGT TTT CCC AGT CAC GAC CAG GAA ACA GCT ATG ACC	24 18 17 24 18	7375 5529 5224 7285 5502	54 48 47 63 50
pGEX primers pGEX 5’ pGEX 3’	GGG CTG GCA AGC CAC GTT TGG TG CCG GGA GCT GCA TGT GTC AGA GG	23 23	7128 7137	62 62
pET primers T7 promoter T7 terminator	TAA TAC GAC TCA CTA TAG GG GCT AGT TAT TGC TCA GCG G	20 19	6122 5830	48 51
pGL/pCAT primers GL primer 1 GL primer 2 RV primer 3 RV primer 4	TGT ATC TTA TGG TAC TGT AAC TG CTT TAT GTT TTT GGC GTC TTC CA CTA GCA AAA TAG GCT GTC CC GAC GAT AGT CAT GCC CCG CG	23 23 20 20	7056 6975 6083 6098	50 52 52 58