Sacchi, Nicoletta, PhD:  Selected Publications (2000-date)

• Sirchia, S. M., Ferguson, A. T., Sironi, E., Subramanyan, S., Orlandi, R., Sukumar, S., and Sacchi, N. (2000). Evidence of epigenetic changes affecting the chromatin state of the retinoic acid receptor beta2 promoter in breast cancer cells. Oncogene 19, 1556-1563.
• Virmani, A. K., Rathi, A., Zochbauer-Muller, S., Sacchi, N., et al. (2000). Promoter methylation and silencing of the retinoic acid receptor-beta gene in lung carcinomas. J Natl Cancer Inst 92, 1303-1307.
• Wood, J. D., Nucifora, F. C., Jr., Duan, K., Zhang, C., Wang, J., Kim, Y., Schilling, G., Sacchi, N., et al (2000). Atrophin-1, the dentato-rubral and pallido-luysian atrophy gene product, interacts with ETO/MTG8 in the nuclear matrix and represses transcription. J Cell Biol 150, 939-948.
• Evron, E., Dooley, W. C., Umbricht, C. B., Rosenthal, D., Sacchi, N., et al (2001). Detection of breast cancer cells in ductal lavage fluid by methylation-specific PCR. Lancet 357, 1335-1336.
• Sacchi, N., Schonkeren, J., Fenaroli, A., Schiaffonati, L., Stoyanova, V., Rossetti, S., Van Unen, L., and Hoogeveen, A. T. (2001). Evidence of different repertoires of MTG (ETO/CDR) oncoproteins in human myeloid leukemia. Am J Hum Genet 69S, 262.
• Sirchia, S. M., Sironi, E., Somenzi, G., Pili, R., Pozzi, S., and Sacchi, N. (2001). Targeting epigenetic changes associated with retinoid resistance: a novel strategy to treat breast cancer. Am J Hum Genet 69S, 205.
• Hoogeveen, A. T., Rossetti, S., Stoyanova, V., Schonkeren, J., Fenaroli, A., Schiaffonati, L., van Unen, L., and Sacchi, N. (2002). The transcriptional corepressor MTG16a contains a novel nucleolar targeting sequence deranged in t (16; 21)-positive myeloid malignancies. Oncogene 21, 6703-6712.
• Sirchia, S. M., Ren, M., Pili, R., Sironi, E., Somenzi, G., Ghidoni, R., Toma, S., Nicolo, G., and Sacchi, N. (2002). Endogenous reactivation of the RARbeta2 tumor suppressor gene epigenetically silenced in breast cancer. Cancer Res 62, 2455-2461.
• Pozzi, S., Ren, M., Somenzi, G., Bistulfi, G., Ghidoni, R., Bosari, S., Sirchia, S. M., and Sacchi, N. (2003). Development of epigenetic resistance to retinoic acid in breast cancer. Implications for chemoprevention. Cancer Epidem Biomar 12, 1318S.
• Somenzi, G., Ren, M., Sala, G., Ghidoni, R., and Sacchi, N. (2003). Impairment of retinoic acid receptor-ceramide signaling in retinoic acid-resistant breast cancer cells. Implications for retinoid differentiation therapy of breast cancer. J Nutr 133, 3852S.
• Bistulfi, G., Ren, M., Pozzi, S., Somenzi, G., and Sacchi, N. (2004). Induction of RARB2 epigenetic silencing leads to development of retinoic acid resistance in cancer cells. J Nutr 134, 3545S.
• Mehrotra, J., Vali, M., McVeigh, M., Kominsky, S. L., Fackler, M. J., Lahti-Domenici, J., Polyak, K., Sacchi, N., et al. (2004). Very high frequency of hypermethylated genes in breast cancer metastasis to the bone, brain, and lung. Clin Cancer Res 10, 3104-3109.
• Rossetti, S., Hoogeveen, A. T., and Sacchi, N. (2004). The MTG proteins: chromatin repression players with a passion for networking. Genomics 84, 1-9.
• Qian, D. Z., Ren, M., Wei, Y., Wang, X., van de Geijn, F., Rasmussen, C., Nakanishi, O., Sacchi, N., et al. (2005). In vivo imaging of retinoic acid receptor beta2 transcriptional activation by the histone deacetylase inhibitor MS-275 in retinoid-resistant prostate cancer cells. Prostate 64, 20-28.
• Ren, M., Pozzi, S., Bistulfi, G., Somenzi, G., Rossetti, S., and Sacchi, N. (2005). Impaired retinoic acid (RA) signal leads to RARbeta2 epigenetic silencing and RA resistance. Mol Cell Biol 25, 10591-10603.
• Rossetti, S., Van Unen, L., Touw, I. P., Hoogeveen, A. T., and Sacchi, N. (2005). Myeloid maturation block by AML1-MTG16 is associated with Csf1r epigenetic downregulation. Oncogene 24, 5325-5332.
• Pozzi, S., Rossetti, S., Bistulfi, G., and Sacchi, N. (2006). RAR-mediated epigenetic control of the cytochrome P450 Cyp26a1 in embryocarcinoma cells. Oncogene 25, 1400-1407.
• Bistulfi, G., Pozzi, S., Ren, M., Rossetti, S., and Sacchi, N. (2006). A repressive epigenetic domino effect confers susceptibility to breast epithelial cell transformation: implications for predicting breast cancer risk. Cancer Res 66, 10308-10314.
• Chomczynski, P., and Sacchi, N. (2006). The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Twenty something years on. Nature Protocols 1, 581-585.
• Rossetti S, Hoogeveen AT, Liang P, Stanciu C, van der Spek P, Sacchi N.  A distinct epigenetic signature at targets of a leukemia protein.  BMC Genomics 8:38, 2007. 
• Somenzi G, Sala G, Rossetti S, Ren M, Ghidoni R, Sacchi N.  Disruption of retinoic acid receptor alpha reveals the growth promoter face of retinoic acid.  PLoS ONE 2:9:E836, 2007.